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用于通过溶胶-凝胶合成固定化微生物细胞的生物相容性二氧化硅-聚乙二醇基复合材料。

Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis.

作者信息

Lavrova Daria G, Zvonarev Anton N, Alferov Valery A, Khonina Tat'yana G, Shadrina Elena V, Alferov Sergey V, Ponamoreva Olga N

机构信息

Biotechnology Department, Tula State University 1, 300012 Tula, Russia.

Federal Research Center "Pushchino Scientific Centre of Biological Research", G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Russia.

出版信息

Polymers (Basel). 2023 Jan 15;15(2):458. doi: 10.3390/polym15020458.

DOI:10.3390/polym15020458
PMID:36679338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9866734/
Abstract

Biocatalysts based on the methylotrophic yeast VKM Y-2559 immobilized in polymer-based nanocomposites for the treatment of methanol-containing wastewater were developed. The organosilica composites with different matrix-to-filler ratios derived from TEOS/MTES in the presence of PEG (SP-composite) and from silicon-polyethylene glycol (STP-composite) differ in the structure of the silicate phase and its distribution in the composite matrix. Methods of fluorescent and scanning microscopy first confirmed the formation of an organosilica shell around living yeast cells during sol-gel bio-STP-composite synthesis. Biosensors based on the yeast cells immobilized in STP- and SP-composites are characterized by effective operation: the coefficient of sensitivity is 0.85 ± 0.07 mgO × min × mmol and 0.87 ± 0.05 mgO × min × mmol, and the long-term stability is 10 and 15 days, respectively. The encapsulated microbial cells are protected from UV radiation and the toxic action of heavy metal ions. Biofilters based on the developed biocatalysts are characterized by high effectiveness in the utilization of methanol-rich wastewater-their oxidative power reached 900 gO/(m × cycle), and their purification degree was up to 60%.

摘要

开发了基于固定在聚合物基纳米复合材料中的甲基营养酵母VKM Y - 2559的生物催化剂，用于处理含甲醇废水。由TEOS/MTES在PEG存在下衍生的不同基质与填料比的有机硅复合材料（SP复合材料）以及由硅 - 聚乙二醇衍生的复合材料（STP复合材料）在硅酸盐相的结构及其在复合基质中的分布方面存在差异。荧光显微镜和扫描显微镜方法首先证实了在溶胶 - 凝胶生物STP复合材料合成过程中，活酵母细胞周围形成了有机硅壳。基于固定在STP和SP复合材料中的酵母细胞的生物传感器具有有效运行的特点：灵敏度系数分别为0.85±0.07 mgO×min×mmol和0.87±0.05 mgO×min×mmol，长期稳定性分别为10天和15天。封装的微生物细胞受到保护，免受紫外线辐射和重金属离子的毒性作用。基于所开发生物催化剂的生物滤池在富含甲醇废水的利用方面具有高效性——其氧化能力达到900 gO/(m×循环)，净化程度高达60%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/a0513f73734a/polymers-15-00458-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/e0be1b3f8d40/polymers-15-00458-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/977eed1ac171/polymers-15-00458-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/cdcceaa80ec6/polymers-15-00458-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/63fe71cf7dba/polymers-15-00458-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/98e0628b21d8/polymers-15-00458-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/98c779bc3f10/polymers-15-00458-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/5c00ec83185a/polymers-15-00458-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/985e9eab011b/polymers-15-00458-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/ec36d48b8713/polymers-15-00458-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/a0513f73734a/polymers-15-00458-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/e0be1b3f8d40/polymers-15-00458-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/977eed1ac171/polymers-15-00458-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/cdcceaa80ec6/polymers-15-00458-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/63fe71cf7dba/polymers-15-00458-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/98e0628b21d8/polymers-15-00458-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/98c779bc3f10/polymers-15-00458-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/5c00ec83185a/polymers-15-00458-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/985e9eab011b/polymers-15-00458-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/ec36d48b8713/polymers-15-00458-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91af/9866734/a0513f73734a/polymers-15-00458-g010.jpg

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