Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia (UTM), 81310 UTM Skudai, Johor Bahru, Johor, Malaysia.
Department of Chemical Engineering, College of Engineering, King Saud University, Riyadh 11421, Saudi Arabia; King Salman Center for Disability Research, Riyadh 11614, Saudi Arabia.
Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127169. doi: 10.1016/j.ijbiomac.2023.127169. Epub 2023 Sep 30.
The development of advanced multifunctional wound dressings remains a major challenge. Herein, a novel multilayer (ML) electrospun nanofibers (NFs) wound dressing based on diethylenetriamine (DETA) functionalized polyacrylonitrile (PAN), TiO nanoparticles (NPs) coating (Ct), and bioderived gelatin (Gel) was developed for potential applications in wound healing. The ML PAN-DETA-Ct-Gel membrane was developed by combining electrospinning, chemical functionalization, synthesis, and electrospray techniques, using a layer-by-layer method. The ML PAN-DETA-Ct-Gel membrane is comprised of an outer layer of PAN-DETA as a barrier to external microorganisms and structural support, an interlayer TiO NPs (Ct) as antibacterial function, and a contact layer (Gel) to improve biocompatibility and cell viability. The NFs membranes were characterized by scanning electron microscopy (SEM), surface profilometry, BET analysis, and water contact angle techniques to investigate their morphology, surface roughness, porosity, and wettability. The ML PAN-DETA-Ct-Gel wound dressing exhibited good surface roughness, porosity, and better wettability. Cell morphology, proliferation, and viability were determined using fibroblasts (3T3), and antibacterial assays were performed against six pathogens. The ML PAN-DETA-Ct-Gel NFs membrane showed good cell morphology, proliferation, viability, and antibacterial activity compared with other membranes. This new class of ML NFs membranes offers a multifunctional architecture with adequate biocompatibility, cell viability, and antibacterial activity.
先进多功能伤口敷料的开发仍然是一个主要挑战。在此，开发了一种新型多层（ML）电纺纳米纤维（NFs）伤口敷料，基于二乙烯三胺（DETA）功能化聚丙烯腈（PAN）、TiO 纳米粒子（NPs）涂层（Ct）和生物衍生明胶（Gel），用于潜在的伤口愈合应用。ML PAN-DETA-Ct-Gel 膜是通过结合静电纺丝、化学功能化、合成和电喷雾技术，使用层层法开发的。ML PAN-DETA-Ct-Gel 膜由 PAN-DETA 外层作为外部微生物和结构支撑的屏障、中间层 TiO NPs（Ct）作为抗菌功能和接触层（Gel）组成，以提高生物相容性和细胞活力。通过扫描电子显微镜（SEM）、表面轮廓仪、BET 分析和水接触角技术对 NFs 膜进行了表征，以研究其形态、表面粗糙度、孔隙率和润湿性。ML PAN-DETA-Ct-Gel 伤口敷料表现出良好的表面粗糙度、孔隙率和更好的润湿性。使用成纤维细胞（3T3）测定细胞形态、增殖和活力，并对六种病原体进行了抗菌试验。与其他膜相比，ML PAN-DETA-Ct-Gel NFs 膜显示出良好的细胞形态、增殖、活力和抗菌活性。这种新的 ML NFs 膜类提供了一种具有足够生物相容性、细胞活力和抗菌活性的多功能架构。
