Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Medical Technologies, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Oncology, Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Sci Rep. 2020 Feb 20;10(1):3063. doi: 10.1038/s41598-020-59931-2.
One-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. In the present study, polycaprolactone/gelatin (PCL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (PU/EEP). The PU/EEP membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells' adhesion and proliferation. The bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers' diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats' skin wound model. Taking together, the PU/EEP-PCL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities.
单层伤口敷料因其各自的特性和缺点不能满足所有临床需求。因此，由两层具有不同性能的材料组成的双层伤口敷料引起了广泛关注。在本研究中，聚己内酯/明胶（PCL/Gel）支架被电纺在由聚氨酯和蜂胶乙醇提取物（PU/EEP）组成的致密膜上。PU/EEP 膜用作顶层，以保护伤口区域免受外部污染和脱水，而 PCL/Gel 支架用作亚层，以促进细胞的黏附和增殖。对双层伤口敷料的微观结构、力学性能、表面润湿性、抗菌活性、生物降解性、生物相容性以及在动物伤口模型中的疗效和组织病理学分析进行了研究。扫描电子显微镜图像显示 PCL/Gel 支架具有均匀的形态和无珠状结构，平均纤维直径为 237.3±65.1nm。抑菌圈试验表明，该敷料对金黄色葡萄球菌（5.4±0.3mm）、大肠杆菌（1.9±0.4mm）和表皮葡萄球菌（1.0±0.2mm）具有显著的抗菌活性。双层伤口敷料具有较高的亲水性（51.1±4.9°）、生物降解性和生物相容性。双层伤口敷料可显著促进 Wistar 大鼠皮肤伤口模型中伤口的闭合和胶原沉积。综上所述，由于具有优异的力学性能、生物相容性、抗菌性能和伤口愈合活性，PU/EEP-PCL/Gel 双层伤口敷料有望成为生物医学应用的候选材料。
