Meat Science and Animal Biologics Discovery Program, Department of Animal and Dairy Sciences, University of Wisconsin, Madison, Wisconsin, USA.
Egg Safety and Quality Research Unit, USDA-ARS U.S. National Poultry Research Center, Athens, Georgia, USA.
J Environ Sci Health B. 2023;58(11):671-678. doi: 10.1080/03601234.2023.2264742. Epub 2023 Oct 12.
growth kinetic parameters can be used to refine the sensitivity and efficiency of microbial growth-based methods. Therefore, the aim of this study was to construct growth curves for , , and in pure culture and calculate growth kinetics for each species in the same environmental conditions. , and were grown over 48 h and inoculated into 15 mL Hungate tubes ( = 3 trials per species; 5 biological replicates per trial; 3 species; 1 strain per species). Absorbance measurements were taken in 45 min intervals over 24 h. Optical density readings were plotted versus time to calculate growth kinetic parameters. exhibited the longest lag phase ( < 0.001) at 15 h 20 min ± 30 min, versus at 11 h 15 min ± 17 min, and at 9 h 27 min ± 15 min. The exponential phase duration was no longer than 5 h for all species, and doubling times were all less than 1h 30 min. The variation in growth kinetics for the three species of illustrates the importance of determining individual spp. growth responses for optimizing detection based on low bacterial levels. This study provides kinetics and estimates to define enrichment times necessary for low concentration detection.
生长动力学参数可用于提高基于微生物生长的方法的灵敏度和效率。因此，本研究的目的是构建纯培养物中 、 、 和 的生长曲线，并在相同的环境条件下计算每种 物种的生长动力学。 将 和 培养 48 小时，并接种到 15 mL 亨格特管中（每种物种 3 次试验；每次试验 5 个生物学重复；3 个物种；每个物种 1 株）。在 24 小时内以 45 分钟的间隔进行吸光度测量。绘制光密度读数与时间的关系，以计算生长动力学参数。 表现出最长的潜伏期（<0.001），为 15 小时 20 分钟±30 分钟，而 为 11 小时 15 分钟±17 分钟， 为 9 小时 27 分钟±15 分钟。所有物种的指数生长期均不超过 5 小时，倍增时间均小于 1 小时 30 分钟。三种 的生长动力学的变化说明了确定单个 spp.生长反应对于基于低细菌水平优化检测的重要性。本研究提供了动力学和估计值，以定义低浓度 检测所需的富集时间。
