Ceckler T L, Bryant R G, Penney D P, Gibson S L, Hilf R
Biochem Biophys Res Commun. 1986 Oct 15;140(1):273-9. doi: 10.1016/0006-291x(86)91086-7.
31P-Nuclear magnetic resonance was used to monitor in situ phosphorus containing compounds in mammary tumors after photodynamic therapy, consisting of administration of hematoporphyrin derivative followed by photoradiation of the lesion. A rapid decrease in ATP along with an increase in Pi resonance intensities was observed. The beta-ATP/Pi ratio decreased by 1 hour, dropping in 2 to 8 hours to 0 to 20 percent of that found prior to photoradiation. Disrupted cells and pycnotic nuclei were observed 48 to 72 hours after photoradiation to a depth of approximately 5 mm. Together with previous studies in vitro, reduction in tumor ATP levels appears to be an early biochemical response to photodynamic therapy.
采用31P核磁共振技术监测光动力治疗后乳腺肿瘤中含磷化合物的原位情况，光动力治疗包括给予血卟啉衍生物，随后对病变部位进行光辐射。观察到ATP迅速减少，同时Pi共振强度增加。β-ATP/Pi比值在1小时内下降，在2至8小时内降至光辐射前水平的0%至20%。光辐射至约5毫米深度后48至72小时，观察到细胞破裂和核固缩。与之前的体外研究一起，肿瘤ATP水平的降低似乎是光动力治疗的早期生化反应。
