Endocrine Hospital, Nghe An, Vietnam.
Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.
Int J Med Sci. 2023 Aug 28;20(10):1316-1325. doi: 10.7150/ijms.84899. eCollection 2023.
Micro RNAs (miRs) expression is involved in the pathogenesis of type 2 diabetes mellitus (T2DM). This study investigates the expression levels of plasma miR-29a, miR-146a, and miR-147b and their correlations with clinical parameters in patients with T2DM. 105 patients with T2DM who categorized either as newly diagnosed T2DM (n=52) or treated T2DM (n=53) and 93 healthy individuals were included in this study. The expression levels of miR-29a, miR-146a, and miR-147b were quantified by real-time PCR and analyzed for possible association with T2DM. The expressions of miR-29a and miR-147b were significantly increased in T2DM patients compared with healthy controls (<0.0001). The expression levels of miR-29a in newly diagnosed T2DM patients were higher than that in the group of treated T2DM (=0.002). The expression of studied miRs was correlated with several clinical parameters such as blood glucose levels, HbA1C, microalbuminuria, C-peptide, triglyceride levels as well as the HOMA-β index. The expression levels of miR-29a and miR-147b show a potential diagnostic performance to discriminate newly diagnostic T2DM (AUCs=0.77 and 0.84, respectively) and beta-cell dysfunction (AUCs= 0.62 and 0.75, respectively). The plasma miR-29a and miR-147b expression levels in T2DM patients are significantly associated with T2DM while miR-146a shows poor evidence in relation to T2DM. miR-147b shows potential as a biomarker for the diagnosis of T2DM and pancreatic beta cell dysfunction.
微小 RNA（miRs）的表达参与 2 型糖尿病（T2DM）的发病机制。本研究探讨了血浆 miR-29a、miR-146a 和 miR-147b 的表达水平及其与 T2DM 患者临床参数的相关性。本研究纳入了 105 例 T2DM 患者，分为新诊断的 T2DM（n=52）和治疗的 T2DM（n=53）以及 93 名健康个体。通过实时 PCR 定量检测 miR-29a、miR-146a 和 miR-147b 的表达水平，并分析其与 T2DM 的可能相关性。与健康对照组相比，T2DM 患者的 miR-29a 和 miR-147b 表达显著升高（<0.0001）。新诊断的 T2DM 患者的 miR-29a 表达水平高于治疗组（=0.002）。研究的 miRs 的表达与血糖水平、HbA1C、微量白蛋白尿、C-肽、甘油三酯水平以及 HOMA-β 指数等多种临床参数相关。miR-29a 和 miR-147b 的表达水平具有潜在的诊断性能，可区分新诊断的 T2DM（AUCs 分别为 0.77 和 0.84）和β细胞功能障碍（AUCs 分别为 0.62 和 0.75）。T2DM 患者血浆 miR-29a 和 miR-147b 的表达水平与 T2DM 显著相关，而 miR-146a 与 T2DM 的相关性证据不足。miR-147b 可能成为 T2DM 和胰岛β细胞功能障碍的诊断标志物。
