Lycheva T A, Petrovskaia V G
Zh Mikrobiol Epidemiol Immunobiol. 1979 May(5):72-8.
Episome F'13 introduced into the genome of a virulent Sh. flexneri strain brought about changes in a number of properties of the recipient strain. The expression of these properties was not connected with the chromosome area allelic to the plasmid genome. These changes seem to be induced by the mobilization of the chromosome genes of E. coli. The loss of virulence in Sh. flexneri strains carrying episome F'13 seemed to be the consequence of two reasons: the overlapping of kcpA gene by its dominant avirulent allele and abnormal synthesis of cell wall lipopolysaccharide due to the transfer of the mobilized genes from the donor strain F'13. When the preliminary mapping of genes on the chromomome was made with the use of plasmids, it was found necessary to use F-episomes which had no influence on the changes occurring in the phenotypic characteristics of the recipient.
导入强毒株弗氏志贺氏菌基因组中的附加体F'13使受体菌株的许多特性发生了变化。这些特性的表达与质粒基因组的等位染色体区域无关。这些变化似乎是由大肠杆菌染色体基因的动员诱导的。携带附加体F'13的弗氏志贺氏菌菌株毒力的丧失似乎是两个原因导致的：kcpA基因被其显性无毒等位基因重叠，以及由于来自供体菌株F'13的可动员基因转移导致细胞壁脂多糖合成异常。当使用质粒对染色体上的基因进行初步定位时，发现有必要使用对受体表型特征变化没有影响的F-附加体。
