Sidhu G S, Dhaunsi G S, Garg U C, Bhatnagar R
Biochem Med Metab Biol. 1986 Oct;36(2):179-85. doi: 10.1016/0885-4505(86)90122-2.
The effect of dietary cholesterol load on the reabsorption of nutrients and the enzyme and chemical characteristics of renal brush-border membrane (BBM) was evaluated in guinea pigs. The transport of D-glucose and amino acids into the renal BBM vesicles of experimental animals was significantly (P less than 0.001) decreased. Vmax of leucine aminopeptidase decreased without alteration in Km; however, both the Km and Vmax of alkaline phosphatase and maltase decreased in renal membrane in response to cholesterol load. The alterations in the chemical architecture of the membrane could possibly be responsible for the observed aberrations in the kidneys of cholesterol-fed animals.
在豚鼠身上评估了饮食胆固醇负荷对营养物质重吸收以及肾刷状缘膜（BBM）的酶和化学特性的影响。实验动物肾BBM囊泡中D-葡萄糖和氨基酸的转运显著降低（P小于0.001）。亮氨酸氨肽酶的Vmax降低而Km未改变；然而，碱性磷酸酶和麦芽糖酶的Km和Vmax在肾膜中均因胆固醇负荷而降低。膜化学结构的改变可能是胆固醇喂养动物肾脏中观察到的异常现象的原因。
