Bioprocess development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City for Scientific Research and Technology Applications, New Borg El-Arab, 21934, Alexandria, Egypt.
Science College, Biology Department, Taibah University, 42317- 8599, Al-Madinah Al-Munawara, Kingdom of Saudi Arabia.
Environ Sci Pollut Res Int. 2022 Jun;29(26):39684-39701. doi: 10.1007/s11356-022-18537-z. Epub 2022 Feb 2.
Resistance to antibiotics is on the rise, and its indiscriminate usage has resulted in human and animal management constraints. In the research for an innovative treatment to diminish antimicrobial resistance, lactic acid bacteria (LAB) throw light on diminishing this problem in public health. As a result, this paper looked at the efficacy of LAB isolates and their active metabolites to combat pathogens, reduce antibiotic use in clinical settings, and explore the anticancer potential of 8 strains of LAB isolated from dairy products. Antifungal and antibacterial potential of LAB isolates against selected crop pathogenic fungi and food pathogenic bacteria had been estimated. Results revealed that all isolates exert antioxidant efficacy relating to DPPH, NO scavenging ability, reducing power, superoxide anion, hydroxyl radical, and anti-lipid peroxidation potential. Additionally, 12B isolate exert the highest anticancer upshot with IC values of 43.98 ± 0.4; 36.7 ± 0.6, 43.1 ± 0.8, and 35.1 ± 0.3 μg/ml, versus Caco-2, MCF-7, HepG-2, and PC3 cell lines respectively, whereas 13B isolate significantly had the highest selectivity index between peripheral blood mononuclear cells (PBMCs) and the tested human cancer cell lines compared to 5-fluorouracil. 13B was the most apoptosis-dependent death inducer for all human cancer cell lines besides exerting the lowest percentage of apoptosis against PBMCs suggesting its safety against PBMCs. The most promising strains 12B and 13B were identified by 16S rRNA sequencing as Lactiplantibacillus plantarum ESSG1 (MZ683194.1) and Lactiplantibacillus pentosus ESSG2 (MZ683195.1). LAB and their extracts are superb substitutive, safe, and efficient antimicrobial, antioxidant, and antitumor curative agents.
抗生素耐药性呈上升趋势，其滥用导致人类和动物管理受到限制。在研究创新治疗方法以减少抗生素耐药性的过程中，乳酸菌（LAB）在公共卫生领域为解决这一问题提供了思路。因此，本文研究了 LAB 分离株及其活性代谢物对抗病原体的功效，以减少临床环境中抗生素的使用，并探索了从乳制品中分离出的 8 株 LAB 的抗癌潜力。评估了 LAB 分离株对选定作物病原菌和食源性病原体的抗真菌和抗菌潜力。结果表明，所有分离株均具有抗氧化功效，与 DPPH、NO 清除能力、还原能力、超氧阴离子、羟自由基和抗脂质过氧化能力有关。此外，12B 分离株表现出最高的抗癌效果，IC 值分别为 43.98±0.4；36.7±0.6、43.1±0.8 和 35.1±0.3μg/ml，对 Caco-2、MCF-7、HepG-2 和 PC3 细胞系；而 13B 分离株与 5-氟尿嘧啶相比，在人外周血单核细胞（PBMC）和测试的人癌细胞系之间具有最高的选择性指数。13B 是所有人类癌细胞系中最依赖于凋亡的死亡诱导剂，除了对 PBMCs 的凋亡率最低外，这表明其对 PBMCs 的安全性。通过 16S rRNA 测序，最有前途的菌株 12B 和 13B 被鉴定为植物乳杆菌 ESSG1（MZ683194.1）和戊糖片球菌 ESSG2（MZ683195.1）。LAB 及其提取物是极好的替代物，具有安全、高效的抗菌、抗氧化和抗肿瘤治疗作用。
