College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, PR China; School of Pharmaceutical Science, Health Science Center, Shenzhen University, Shenzhen 518060, PR China; Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.
School of Pharmaceutical Science, Health Science Center, Shenzhen University, Shenzhen 518060, PR China; Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan.
Food Chem. 2022 May 30;377:131954. doi: 10.1016/j.foodchem.2021.131954. Epub 2021 Dec 27.
An exopolysaccharide, designated as MM89-EPS, was isolated from Lactiplantibacillus plantarum MM89. It was comprised of glucose and mannose molecules with an average molecular weight of 138 kDa. FTIR and NMR spectra showed that MM89-EPS had characteristic polysaccharide functional groups. MM89-EPS displayed excellent water solubility and capacities to retain water and oil due to its porous structure. MM89-EPS exhibited no significant cytotoxicity on RAW264.7 cells and showed strong immunomodulatory activity by increasing phagocytosis, acid phosphatase activity, and cytokine production in RAW264.7 cells. Furthermore, an in vivo study revealed that splenic indices, intestinal IgA levels, serum cytokine levels, and lymphocyte proliferation were increased in an MM89-EPS-treated cyclophosphamide-induced immunosuppressed mouse model. To summarize, our results indicate that MM89-EPS can efficiently enhance the immunostimulatory activity of immune cells and an immunosuppressed mouse model. Hence, MM89-EPS may be use as a potential source of immunomodulatory agent in various food products.
从植物乳杆菌 MM89 中分离得到一种胞外多糖，命名为 MM89-EPS。它由葡萄糖和甘露糖分子组成，平均分子量为 138 kDa。傅里叶变换红外光谱（FTIR）和核磁共振（NMR）图谱表明 MM89-EPS 具有特征性多糖官能团。由于其多孔结构，MM89-EPS 具有优异的水溶性和保水、保油性。MM89-EPS 对 RAW264.7 细胞没有明显的细胞毒性，并通过增加 RAW264.7 细胞的吞噬作用、酸性磷酸酶活性和细胞因子产生，表现出强烈的免疫调节活性。此外，体内研究表明，在环磷酰胺诱导的免疫抑制小鼠模型中，MM89-EPS 处理可增加脾脏指数、肠道 IgA 水平、血清细胞因子水平和淋巴细胞增殖。总之，我们的研究结果表明，MM89-EPS 可有效增强免疫细胞和免疫抑制小鼠模型的免疫刺激活性。因此，MM89-EPS 可能可作为各种食品中免疫调节剂的潜在来源。
