Wei Xiaoliao, Fu Tianling, He Guandi, Zhong Zhuoyan, Yang Mingfang, Lou Fei, He Tengbing
College of Agriculture, Guizhou University, Guiyang, China.
Engineering Key Laboratory for Pollution Control and Resource Reuse Technology of Mountain Livestock Breeding, Institute of New Rural Development, Guizhou University, Guiyang, China.
Front Microbiol. 2023 Sep 15;14:1241436. doi: 10.3389/fmicb.2023.1241436. eCollection 2023.
Understanding the rhizosphere soil microbial community and its relationship with the bulk soil microbial community is critical for maintaining soil health and fertility and improving crop yields in regions. The microbial communities in the rhizosphere and bulk soils of a Chinese cabbage () plantation in a region, as well as their relationships with soil nutrients, were examined in this study using high-throughput sequencing technologies of 16S and ITS amplicons. The aim was to provide theoretical insights into the healthy cultivation of Chinese cabbage in a area. The findings revealed that the rhizosphere soil showed higher contents of organic matter (OM), alkaline hydrolyzable nitrogen (AN), available phosphorus (AP), total phosphorus (TP), available potassium (AK), total potassium (TK), total nitrogen (TN), catalase (CA), urease (UR), sucrase (SU), and phosphatase (PHO), in comparison with bulk soil, while the pH value showed the opposite trend. The diversity of bacterial and fungal communities in the bulk soil was higher than that in the rhizosphere soil, and their compositions differed between the two types of soil. In the rhizosphere soil, Proteobacteria, Acidobacteriota, Actinobacteriota, and Bacteroidota were the dominant bacterial phyla, while Olpidiomycota, Ascomycota, Mortierellomycota, and Basidiomycota were the predominant fungal phyla. In contrast, the bulk soil was characterized by bacterial dominance of Proteobacteria, Acidobacteriota, Chloroflexi, and Actinobacteriota and fungal dominance of Ascomycota, Olpidiomycota, Mortierellomycota, and Basidiomycota. The fungal network was simpler than the bacterial network, and both networks exhibited less complexity in the rhizosphere soil compared with the bulk soil. Moreover, the rhizosphere soil harbored a higher proportion of beneficial Rhizobiales. The rhizosphere soil network was less complicated than the network in bulk soil by building a bacterial-fungal co-occurrence network. Furthermore, a network of relationships between soil properties and network keystone taxa revealed that the rhizosphere soil keystone taxa were more strongly correlated with soil properties than those in the bulk soil; despite its lower complexity, the rhizosphere soil contains a higher abundance of bacteria which are beneficial for cabbage growth compared with the bulk soil.
了解根际土壤微生物群落及其与非根际土壤微生物群落的关系对于维持土壤健康和肥力以及提高该地区的作物产量至关重要。本研究采用16S和ITS扩增子的高通量测序技术,对某地区大白菜种植地的根际和非根际土壤中的微生物群落及其与土壤养分的关系进行了研究。目的是为该地区大白菜的健康种植提供理论依据。研究结果表明,与非根际土壤相比,根际土壤中的有机质(OM)、碱解氮(AN)、有效磷(AP)、总磷(TP)、速效钾(AK)、全钾(TK)、全氮(TN)、过氧化氢酶(CA)、脲酶(UR)、蔗糖酶(SU)和磷酸酶(PHO)含量更高,而pH值则呈现相反的趋势。非根际土壤中细菌和真菌群落的多样性高于根际土壤,且两种土壤类型中群落组成存在差异。在根际土壤中,变形菌门、酸杆菌门、放线菌门和拟杆菌门是主要的细菌门类,而卵菌门、子囊菌门、被孢霉门和担子菌门是主要的真菌门类。相比之下,非根际土壤的特征是变形菌门、酸杆菌门、绿弯菌门和放线菌门占主导地位的细菌群落以及子囊菌门、卵菌门、被孢霉门和担子菌门占主导地位的真菌群落。真菌网络比细菌网络更简单,与非根际土壤相比,两种网络在根际土壤中的复杂性都较低。此外,根际土壤中有益的根瘤菌目比例更高。通过构建细菌-真菌共现网络,根际土壤网络比非根际土壤网络更简单。此外,土壤性质与网络关键类群之间的关系网络表明,根际土壤关键类群与土壤性质的相关性比非根际土壤更强;尽管根际土壤复杂性较低,但与非根际土壤相比,其含有更丰富的对白菜生长有益的细菌。