涡虫前后轴特化过程中单碱基水平上m6A动态变化的解析。
Deciphering m6A dynamics at a single-base level during planarian anterior-posterior axis specification.
作者信息
Chen Liqian, Zhen Hui, Chen Zixin, Huang Mujie, Mak Daniel W, Jin Wei, Zou Yuxiu, Chen Mingjie, Zheng Mingyue, Xie Qingqiang, Zhou Zhongjun, Jin Guoxiang
机构信息
Guangdong Cardiovascular Institute, Medical Research Institute, Guangdong Provincial Geriatrics Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China.
School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
出版信息
Comput Struct Biotechnol J. 2023 Sep 18;21:4567-4579. doi: 10.1016/j.csbj.2023.09.018. eCollection 2023.
BACKGROUND
The establishment of the anterior-posterior (A-P) axis is a crucial step during tissue repair and regeneration. Despite the association reported recently of -methyladenosine (m6A) with regeneration, the mechanism underlying the regulation of m6A in A-P axis specification during regeneration remains unknown. Herein, we deciphered the m6A landscape at a single-base resolution at multiple time points during A-P axis regeneration and constructed the transcriptome assembly of the planarian.
RESULTS
Immunofluorescence staining and comparative analysis revealed that m6A is widespread across the planarian and dynamically regulated during regeneration along the A-P axis, exhibiting a strong spatiotemporal feature. The resulting datasets of m6A-modified genes identified 80 anterior-specific genes and 13 posterior-specific genes, respectively. In addition, we showed that YTHDC1 serves as the primary m6A reader to be involved in the m6A-mediated specification of A-P axis during regeneration in planarian.
CONCLUSIONS
Our study provides an RNA epigenetic explanation for the specification of the A-P axis during tissue regeneration in planarian.
背景
前后轴（A-P轴）的建立是组织修复和再生过程中的关键步骤。尽管最近报道了N6-甲基腺苷（m6A）与再生有关，但在再生过程中m6A调控A-P轴特化的潜在机制仍不清楚。在此，我们在A-P轴再生的多个时间点以单碱基分辨率解析了m6A图谱，并构建了涡虫的转录组组装。
结果
免疫荧光染色和比较分析表明，m6A在涡虫中广泛存在，并在沿A-P轴的再生过程中受到动态调控，呈现出强烈的时空特征。由此产生的m6A修饰基因数据集分别鉴定出80个前部特异性基因和13个后部特异性基因。此外，我们表明YTHDC1作为主要的m6A阅读器，参与涡虫再生过程中m6A介导的A-P轴特化。
结论
我们的研究为涡虫组织再生过程中A-P轴的特化提供了一种RNA表观遗传学解释。
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