Rachid Ousama, Akkbik Mohammed, Alkilany Alaaldin M, Makhlouf Ahmed, Al Shaikh Loua, Alinier Guillaume
College of Pharmacy, QU Health, Qatar University, Doha, Qatar.
Central Laboratories Unit, Office of VP for Research & Graduate Studies, Qatar University, Doha, Qatar.
Heliyon. 2023 Sep 25;9(10):e20377. doi: 10.1016/j.heliyon.2023.e20377. eCollection 2023 Oct.
Data on stability and suitability to use normal saline stored under stress conditions in ambulances is lacking.
We aimed to study the impact of exposure to extreme temperature variations on normal saline stability and compatibility with its packaging.
Normal saline in 96 polyolefin bags were exposed to continuous temperature of 22, 50, and 70 °C or to a cyclic temperature of 70 °C per 8 h and 22 °C per 16 h. The bags were sampled at 12, 24, 48 and 72 h and at 1, 2, 3, and 4 weeks in the short- and long-term experiments, respectively. Solution inside the bags was evaluated for any evidence of crystallization, discoloration, turbidity, or pH changes. A sample of normal saline was withdrawn from each bag to analyze sodium and chloride levels.
Precipitation, discoloration, or turbidity were not observed in the solution inside normal saline bags. The average pH was 5.59 at 22 °C, 5.73 at 50 °C, 5.86 at 70 °C and 5.79 at cyclic exposure. In the short- and long-term experiments, sodium and chloride concentrations were within 100.2-111.27% and 99.04-110.95%, respectively. Leaching of the plastic components in the polyolefin bag into the normal saline solution was not detected.
Sodium and chloride levels of normal saline were stable and compatible with polyolefin bags stored in simulated continuous and cyclic extreme temperatures for around one month. The effect of storage in the cabinet of operational ambulance vehicles during different seasons in arid countries is yet to be evaluated in real-world conditions, to further confirm our results.
缺乏关于在救护车压力条件下储存的生理盐水稳定性和适用性的数据。
我们旨在研究暴露于极端温度变化对生理盐水稳定性及其与包装兼容性的影响。
将96个聚烯烃袋中的生理盐水暴露于22、50和70°C的持续温度下，或每8小时70°C和每16小时22°C的循环温度下。在短期和长期实验中，分别在12、24、48和72小时以及1、2、3和4周时对袋子进行取样。评估袋内溶液是否有结晶、变色、浑浊或pH值变化的迹象。从每个袋子中取出一份生理盐水样本，分析钠和氯的水平。
在生理盐水袋内的溶液中未观察到沉淀、变色或浑浊。22°C时平均pH值为5.59，50°C时为5.73，70°C时为5.86，循环暴露时为5.79。在短期和长期实验中，钠和氯的浓度分别在100.2-111.27%和99.04-110.95%之间。未检测到聚烯烃袋中的塑料成分浸出到生理盐水中。
生理盐水的钠和氯水平稳定，与储存在模拟连续和循环极端温度下约一个月的聚烯烃袋兼容。在干旱国家不同季节，运营救护车车厢内储存的影响有待在实际条件下进行评估，以进一步证实我们的结果。
