Department of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14A, Wrocław, Poland.
FEMS Microbiol Rev. 2023 Nov 1;47(6). doi: 10.1093/femsre/fuad057.
Bdellovibrio bacteriovorus, an obligate predatory Gram-negative bacterium that proliferates inside and kills other Gram-negative bacteria, was discovered more than 60 years ago. However, we have only recently begun to understand the detailed cell biology of this proficient bacterial killer. Bdellovibrio bacteriovorus exhibits a peculiar life cycle and bimodal proliferation, and thus represents an attractive model for studying novel aspects of bacterial cell biology. The life cycle of B. bacteriovorus consists of two phases: a free-living nonreplicative attack phase and an intracellular reproductive phase. During the reproductive phase, B. bacteriovorus grows as an elongated cell and undergoes binary or nonbinary fission, depending on the prey size. In this review, we discuss: (1) how the chromosome structure of B. bacteriovorus is remodeled during its life cycle; (2) how its chromosome replication dynamics depends on the proliferation mode; (3) how the initiation of chromosome replication is controlled during the life cycle, and (4) how chromosome replication is spatiotemporally coordinated with the proliferation program.
蛭弧菌，一种专性捕食的革兰氏阴性菌，可在体内增殖并杀死其他革兰氏阴性菌，早在 60 多年前就被发现了。然而，我们直到最近才开始了解这种高效细菌杀手的详细细胞生物学。蛭弧菌表现出一种特殊的生命周期和双峰增殖，因此它是研究细菌细胞生物学新方面的一个有吸引力的模型。蛭弧菌的生命周期包括两个阶段：自由生活的非复制攻击阶段和细胞内繁殖阶段。在繁殖阶段，蛭弧菌作为一个拉长的细胞生长，并根据猎物的大小进行二元或非二元分裂。在这篇综述中，我们讨论了：（1）蛭弧菌的染色体结构在其生命周期中是如何重塑的；（2）其染色体复制动力学如何依赖于增殖模式；（3）染色体复制的起始如何在生命周期中受到控制；以及（4）染色体复制如何与增殖程序在时空上协调。
