Max Planck Institute for Terrestrial Microbiology & Center for Synthetic Microbiology (SYNMIKRO) , Marburg, Germany.
Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas , Granada, Spain.
mBio. 2023 Oct 31;14(5):e0209923. doi: 10.1128/mbio.02099-23. Epub 2023 Oct 4.
Chemotaxis of motile bacteria has multiple physiological functions. It enables bacteria to locate optimal ecological niches, mediates collective behaviors, and can play an important role in infection. These multiple functions largely depend on ligand specificities of chemoreceptors, and the number and identities of chemoreceptors show high diversity between organisms. Similar diversity is observed for the spectra of chemoeffectors, which include not only chemicals of high metabolic value but also bacterial, plant, and animal signaling molecules. However, the systematic identification of chemoeffectors and their mapping to specific chemoreceptors remains a challenge. Here, we combined several and approaches to establish a systematic screening strategy for the identification of receptor ligands and we applied it to identify a number of new physiologically relevant chemoeffectors for the important opportunistic human pathogen . This strategy can be equally applicable to map specificities of sensory domains from a wide variety of receptor types and bacteria.
游动细菌的趋化性具有多种生理功能。它使细菌能够定位最佳的生态位，介导群体行为，并在感染中发挥重要作用。这些多种功能在很大程度上取决于化学感受器的配体特异性，并且化学感受器的数量和身份在生物体之间表现出高度的多样性。化学引诱物的光谱也观察到类似的多样性，其中不仅包括高代谢价值的化学物质，还包括细菌、植物和动物的信号分子。然而，化学引诱物的系统鉴定及其与特定化学感受器的映射仍然是一个挑战。在这里，我们结合了几种方法，建立了一种系统的筛选策略，用于鉴定受体配体，并将其应用于鉴定重要的机会性病原体的许多新的生理相关化学引诱物。这种策略同样适用于从各种受体类型和细菌中映射感觉域的特异性。
