Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada.
PLoS One. 2023 Oct 4;18(10):e0292430. doi: 10.1371/journal.pone.0292430. eCollection 2023.
This study evaluated surface properties and adhesion/biofilm formation by Candida albicans on 3D printed denture base resins used in 3D printing. Disc-shaped specimens (15 mm x 3 mm) of two 3D-printed resins (NextDent Denture 3D+, NE, n = 64; and Cosmos Denture, CO, n = 64) and a heat-polymerized resin (Lucitone 550, LU, control, n = 64) were analyzed for surface roughness (Ra μm) and surface free energy (erg cm-2). Microbiologic assays (90-min adhesion and 48-h biofilm formation by C. albicans) were performed five times in triplicate, with the evaluation of the specimens' surface for: (i) colony forming units count (CFU/mL), (ii) cellular metabolism (XTT assay), and (iii) fluorescence and thickness of biofilm layers (confocal laser scanning microscopy). Data were analyzed using parametric and nonparametric tests (α = 0.05). LU presented higher surface roughness Ra (0.329±0.076 μm) than NE (0.295±0.056 μm) (p = 0.024), but both were similar to CO (0.315±0.058 μm) (p = 1.000 and p = 0.129, respectively). LU showed lower surface free energy (47.47±2.01 erg cm-2) than CO (49.61±1.88 erg cm-2) and NE (49.23±2.16 erg cm-2) (p<0.001 for both). The CO and NE resins showed greater cellular metabolism (p<0.001) and CO only, showed greater colonization (p = 0.015) by C. albicans than LU in the 90-min and 48-hour periods. It can be concluded that both 3D-printed denture base resins are more prone to colonization by C. albicans, and that their surface free energy may be more likely associated with that colonization than their surface roughness.
本研究评估了用于 3D 打印的义齿基托树脂的表面性能和白色念珠菌的粘附/生物膜形成。对两种 3D 打印树脂（NextDent 义齿 3D+，NE，n=64；和 Cosmos 义齿，CO，n=64）和一种热聚合树脂（Lucitone 550，LU，对照，n=64）的圆盘状标本（15mm×3mm）进行了表面粗糙度（Ra μm）和表面自由能（erg cm-2）分析。微生物学测定（白色念珠菌 90 分钟粘附和 48 小时生物膜形成）进行了五次重复，评估了标本表面的：（i）菌落形成单位计数（CFU/mL），（ii）细胞代谢（XTT 测定），和（iii）生物膜层的荧光和厚度（共焦激光扫描显微镜）。使用参数和非参数检验（α=0.05）对数据进行分析。LU 的表面粗糙度 Ra（0.329±0.076 μm）高于 NE（0.295±0.056 μm）（p=0.024），但均与 CO（0.315±0.058 μm）相似（p=1.000 和 p=0.129，分别）。LU 的表面自由能（47.47±2.01 erg cm-2）低于 CO（49.61±1.88 erg cm-2）和 NE（49.23±2.16 erg cm-2）（p<0.001）。CO 和 NE 树脂显示出更大的细胞代谢（p<0.001），并且仅 CO 显示出在 90 分钟和 48 小时期间比 LU 对白色念珠菌的更大定植（p=0.015）。可以得出结论，两种 3D 打印义齿基托树脂更容易被白色念珠菌定植，并且它们的表面自由能可能与定植更相关，而不是它们的表面粗糙度。
