Perić Mirjana, Petrović Sanja, Čairović Aleksandra, Vlajić Tovilović Tamara, Racić Aleksandar, Panajotović Radmila, Živković Rade, Miličić Biljana, Radunović Milena
Department of Prosthodontics, School of Dental Medicine, University of Belgrade, Rankeova 4, Belgrade, 11000, Serbia.
Department of Microbiology, School of Dental Medicine, University of Belgrade, Dr. Subotića 1, Belgrade, 11000, Serbia.
Clin Oral Investig. 2025 Aug 28;29(9):423. doi: 10.1007/s00784-025-06488-x.
To evaluate the effects of hydrothermal ageing on biofilm adhesion and surface properties of denture base resins fabricated via different techniques.
Denture base resin samples were produced using the following manufacturing techniques: conventional cold polymerization (CP), heat polymerization (HP), CAD-CAM milling (CADm) and CAD-CAM printing (3D). The samples were tested before and after hydrothermal ageing (5000 cycles, 5 °C/55°C). The surface roughness was measured using atomic force microscopy (AFM), and the hydrophobicity was observed by measuring the water contact angle (WCA). Monomicrobial biofilm of Staphylococcus aureus and Candida albicans were formed and quantified before and after ageing using colony-forming units (CFUs/ml) and the MTT test. Statistical differences were evaluated using a two-way analysis of variance (ANOVA).
Two-way ANOVA showed that ageing significantly influenced the roughness (p = 0.002). The highest roughness values before and after ageing were measured for 3D resin samples. The WCA values of 3D resin samples changed after ageing (p = 0.018). Amount and metabolic activity of monomicrobial biofilms were increased during ageing on all four tested denture base resins.
Ageing influenced the roughness and microbial adhesion on all tested denture base materials, regardless of the manufacturing method.
Finding support replacing dentures every five years due to increased biofilm adhesion post ageing.
评估水热老化对通过不同技术制造的义齿基托树脂生物膜粘附和表面性能的影响。
使用以下制造技术制备义齿基托树脂样品：传统冷聚合法（CP）、热聚合法（HP）、计算机辅助设计与制造铣削法（CADm）和计算机辅助设计与制造打印法（3D）。在水热老化（5000个循环，5°C/55°C）前后对样品进行测试。使用原子力显微镜（AFM）测量表面粗糙度，并通过测量水接触角（WCA）观察疏水性。在老化前后，使用菌落形成单位（CFUs/ml）和MTT试验形成并定量金黄色葡萄球菌和白色念珠菌的单微生物生物膜。使用双向方差分析（ANOVA）评估统计学差异。
双向方差分析表明，老化对粗糙度有显著影响（p = 0.002）。老化前后粗糙度值最高的是3D树脂样品。老化后3D树脂样品的WCA值发生了变化（p = 0.018）。在所有四种测试的义齿基托树脂上，老化过程中单一微生物生物膜的数量和代谢活性均增加。
无论制造方法如何，老化都会影响所有测试义齿基托材料的粗糙度和微生物粘附。
由于老化后生物膜粘附增加，建议每五年更换义齿。
