Kurzendorfer-Brose Laura, Rosentritt Martin
Department of Prosthetic Dentistry, UKR University Hospital Regensburg, 93042 Regensburg, Germany.
Materials (Basel). 2025 Mar 17;18(6):1323. doi: 10.3390/ma18061323.
(1) Understanding the effects of manufacturing factors on microbial adhesion is essential for optimizing additive denture base materials and improving their clinical performance. This study evaluated how polymerization time, layer thickness, extended cleaning, and storage conditions influence and adhesion on a denture base material. (2) Specimens (n = 15/group, d = 8 mm, h = 2 mm) were additively fabricated or poured (reference). Digital light processing was performed with varying polymerization times, layer thicknesses, extended cleaning, and storage. Microbial adhesion was assessed using a luminescence assay. Surface properties were characterized by roughness (S/S), hardness, and surface free energy (SFE). Statistics: The Shapiro-Wilk test, ANOVA, Bonferroni post hoc test, and Pearson correlation (α = 0.05) were utilized. (3) Polymerization time, layer thickness, cleaning, and storage conditions significantly influenced and adhesion. Increased layer thickness reduced adhesion but promoted colonization, emphasizing the role of SFE. Extended polymerization and optimized cleaning reduced microbial adhesion, highlighting the need for tailored processing to enhance microbial resistance and material integrity. (4) Manufacturing factors influenced microbial adhesion, with additive materials reducing the abundance of but increasing the abundance of , underscoring the importance of material adjustments and extended polymerization to enhance microbial resistance.
(1) 了解制造因素对微生物黏附的影响对于优化义齿基托添加剂材料和改善其临床性能至关重要。本研究评估了聚合时间、层厚、延长清洁时间和储存条件如何影响义齿基托材料上的微生物黏附。(2) 制作或浇筑样本（每组n = 15，直径d = 8毫米，高度h = 2毫米）（对照）。采用不同的聚合时间、层厚、延长清洁时间和储存条件进行数字光处理。使用发光测定法评估微生物黏附。通过粗糙度（S/S）、硬度和表面自由能（SFE）来表征表面特性。统计分析：采用夏皮罗-威尔克检验、方差分析、邦费罗尼事后检验和皮尔逊相关性分析（α = 0.05）。(3)聚合时间、层厚、清洁时间和储存条件对微生物黏附和定植有显著影响。增加层厚会降低微生物黏附，但会促进微生物定植，这突出了表面自由能的作用。延长聚合时间和优化清洁可降低微生物黏附，这凸显了进行定制加工以增强抗微生物性和材料完整性的必要性。(4) 制造因素影响微生物黏附，添加剂材料会减少某种微生物的数量，但会增加另一种微生物的数量，这强调了材料调整和延长聚合时间以增强抗微生物性的重要性。
