Aquatic Animal Health Laboratory, Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam 629502, Tamilnadu, India.
Department of Biochemistry, Saveetha Medical College & Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai 602105, Tamilnadu, India.
Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127182. doi: 10.1016/j.ijbiomac.2023.127182. Epub 2023 Oct 2.
Encapsulation of DNA vaccines onto carriers enhances the immunogenicity of an antigen. Specifically, biodegradable polymers offer sustained release of vaccines which is crucial for any targeted delivery approach. Poly (lactic-co-glycolic) acid (PLGA) microspheres were used to load a DNA vaccine having a targeted gene of outer membrane protein (OMP) of Aeromonas hydrophila to clone and construct a DNA vaccine using a eukaryotic expression vector system (pVAX1-OMP DNA) and delivery in Carassius auratus against A. hydrophila infection. PLGA microspheres were prepared by emulsion technique oil-in-water and characterized by a High-Resolution Scanning Electron Microscope (HR-SEM). The results of PLGA-pVAX1-OMP DNA microspheres shows that average of 100-150 μm particle size and a loading efficiency (LE) of 68.8 %. Results indicate that C. auratus fed with PLGA-pVAX1-OMP DNA microspheres revealed a significant improvement in innate immune response, which includes, myeloperoxidase activity, respiratory burst and total immunoglobulin level compared with control group fish. The immune-related gene, IL1β, IL10, TGF, c-type, and g-type lysozyme also showed significantly higher expression after immunization. Furthermore, dietary supplementation of the PLGA-pVAX1-OMP DNA (G III) group exhibited a significantly higher survival rate (78 %) than the control group of fish. These results help us to understand the of mechanism of DNA vaccine administrated feed through PLGA nanoparticles resistance to infection by regulating systemic and innate immunity in Carassius auratus.
将 DNA 疫苗包封在载体上可以增强抗原的免疫原性。具体而言，可生物降解的聚合物提供疫苗的缓释，这对于任何靶向递送方法都是至关重要的。聚（乳酸-共-乙醇酸）（PLGA）微球被用于加载具有气单胞菌外膜蛋白（OMP）靶向基因的 DNA 疫苗，以克隆和构建 DNA 疫苗使用真核表达载体系统（pVAX1-OMP DNA）并在 Carassius auratus 中针对 A. hydrophila 感染进行递送。PLGA 微球通过油包水乳液技术制备，并通过高分辨率扫描电子显微镜（HR-SEM）进行表征。PLGA-pVAX1-OMP DNA 微球的结果表明，粒径为 100-150μm，载药量（LE）为 68.8％。结果表明，与对照组鱼相比，用 PLGA-pVAX1-OMP DNA 微球喂养的 Carassius auratus 表现出先天免疫反应的显著改善，包括髓过氧化物酶活性、呼吸爆发和总免疫球蛋白水平。免疫相关基因，IL1β、IL10、TGF、c 型和 g 型溶菌酶在免疫后也表现出明显更高的表达。此外，PLGA-pVAX1-OMP DNA（G III）组的饮食补充显示出比对照组鱼更高的存活率（78％）。这些结果有助于我们了解 DNA 疫苗通过 PLGA 纳米粒子抵抗感染通过调节系统和 Carassius auratus 先天免疫的机制。
