Ide C
Brain Res. 1986 Oct 8;384(2):311-22. doi: 10.1016/0006-8993(86)91167-4.
Murine Meissner corpuscles (mouse digital corpuscles), located in pad skin at the toe tip, consist of lamellar cells with long cellular processes (lamellae) surrounding axon terminals in an onion-skin fashion. Lamellar cell bodies and processes were provided with a basal lamina. The present study was made to examine whether these lamellar cell basal laminae have any specific role in the differentiation of regenerating axons and Schwann cells into specialized axon terminals and lamellar cells, respectively. Pad skin at the toe tip was treated 3-5 X by freezing and thawing. By this treatment, cellular constituents of the corpuscles die and disintegrate into cell debris, leaving in situ basal laminae of the lamellar cells in stacked hollow loops, reminiscent of the original configuration of lamellae. Schwann cells and axons of the ordinary nerve fibers in the pad skin were similarly damaged, and basal laminae of the Schwann cells remained as basal lamina tubes. Three days after treatment, regenerating axons were seen extending through the basal lamina tubes of Schwann cells deep in the toe pad skin. However, no regenerating axons were found in the vicinity of the old corpuscles. Five days after treatment, regenerating axons, some of which were accompanied by migrating Schwann cells and others which were still naked, were noted at the subepidermal region, and began to enter the hollow basal lamina loops of the old corpuscles. Eight-15 days after treatment, regenerating axons which entered the basal lamina loops successively gave rise to branches, and at the same time, accompanying Schwann cells emanated cellular processes through well-preserved basal lamina loops. Fifteen-25 days after treatment, regenerating axons seemed to be morphologically specialized as axon terminals, and accompanying Schwann cells differentiated into definite lamellar cells which surrounded the axon terminals in the same manner as in the normal murine Meissner corpuscles. Although the incidence of good regeneration of the corpuscle was relatively low, these findings suggested that basal laminae of lamellar cells might have some specific properties which could be responsible for the differentiation as well as maintenance of lamellar cells and axon terminals in the Meissner corpuscles.
小鼠迈斯纳小体（小鼠指端小体）位于趾尖的垫状皮肤中，由具有长细胞突起（板层）的扁平细胞以洋葱皮样方式围绕轴突终末组成。扁平细胞体和突起都有基膜。本研究旨在探讨这些扁平细胞基膜在再生轴突和施万细胞分别分化为特化的轴突终末和扁平细胞过程中是否具有任何特定作用。对趾尖的垫状皮肤进行3 - 5次冻融处理。通过这种处理，小体的细胞成分死亡并分解成细胞碎片，扁平细胞的基膜原位留在堆叠的空心环中，让人联想到板层的原始结构。垫状皮肤中普通神经纤维的施万细胞和轴突也受到类似损伤，施万细胞的基膜保留为基膜管。处理后三天，可见再生轴突穿过趾垫皮肤深处施万细胞的基膜管延伸。然而，在旧小体附近未发现再生轴突。处理后五天，在表皮下区域发现再生轴突，其中一些伴有迁移的施万细胞，另一些仍无髓鞘，并且开始进入旧小体的空心基膜环。处理后8 - 15天，进入基膜环的再生轴突相继发出分支，与此同时，伴随的施万细胞通过保存完好的基膜环发出细胞突起。处理后15 - 25天，再生轴突在形态上似乎特化为轴突终末，伴随的施万细胞分化为明确的扁平细胞，它们以与正常小鼠迈斯纳小体相同的方式围绕轴突终末。尽管小体良好再生的发生率相对较低，但这些发现表明扁平细胞的基膜可能具有一些特定特性，这些特性可能负责迈斯纳小体中扁平细胞和轴突终末的分化以及维持。
