Ide C, Tohyama K, Yokota R, Nitatori T, Onodera S
Brain Res. 1983 Dec 12;288(1-2):61-75. doi: 10.1016/0006-8993(83)90081-1.
Nerve segments approximately 7 mm long were excised from the predegenerated sciatic nerves of mice, and treated 5 times by repetitive freezing and thawing to kill the Schwann cells. Such treated nerve segments were grafted into the original places so as to be in contact with the proximal stumps. The animals were sacrificed 1, 2, 3, 5, 7 and 10 days after the grafting. The grafts were examined by electron microscopy in the middle part of the graft, i.e. 3-4 mm distal to the proximal end and/or near the proximal and distal ends of the graft. In other instances, the predegenerated nerve segments were minced with a razor blade after repetitive freezing and thawing. Such minced nerves were placed in contact with the proximal stumps of the same nerves. The animals were sacrificed 10 days after the grafting. Within 1-2 days after grafting, the dead Schwann cells had disintegrated into fragments. They were then gradually phagocytosed by macrophages. The basal laminae of Schwann cells, which were not attacked by macrophages, remained as empty tubes (basal lamina scaffolds). In the grafts we examined, no Schwann cells survived the freezing and thawing process. The regenerating axons always grew out through such basal lamina scaffolds, being in contact with the inner surface of the basal lamina (i.e. the side originally facing the Schwann cell plasma membrane). No axons were found outside of the scaffolds. One to two days after grafting, the regenerating axons were not associated with Schwann cells, but after 5-7 days they were accompanied by Schwann cells which were presumed to be migrating along axons from the proximal stumps. Ten days after grafting, proliferating Schwann cells observed in the middle part of the grafts had begun to sort out axons. In the grafts of minced nerves, the fragmented basal laminae of the Schwann cells re-arranged themselves into thicker strands or small aggregations of basal laminae. The regenerating axons, without exception, attached to one side of such modified basal laminae. Collagen fibrils were in contact with the other side, indicating that these modified basal laminae had the same polarity in terms of cell attachment as seen in the ordinary basal laminae of the scaffolds.(ABSTRACT TRUNCATED AT 400 WORDS)
从小鼠预先变性的坐骨神经中切下约7毫米长的神经段，通过反复冻融处理5次以杀死雪旺细胞。将经如此处理的神经段移植回原位，使其与近端残端接触。在移植后1、2、3、5、7和10天处死动物。在移植物中部，即距近端3 - 4毫米处和/或移植物近端及远端附近，通过电子显微镜检查移植物。在其他情况下，预先变性的神经段经反复冻融后用剃须刀片切碎。将切碎的神经与同一条神经的近端残端接触。在移植后10天处死动物。移植后1 - 2天内，死亡的雪旺细胞已解体成碎片，随后逐渐被巨噬细胞吞噬。未被巨噬细胞攻击的雪旺细胞基膜作为空管（基膜支架）保留下来。在我们检查的移植物中，没有雪旺细胞在冻融过程中存活。再生轴突总是通过此类基膜支架生长出来，与基膜的内表面（即原本面向雪旺细胞质膜的一侧）接触。在支架之外未发现轴突。移植后1 - 2天，再生轴突未与雪旺细胞相关联，但5 - 7天后，它们伴随着推测是从近端残端沿轴突迁移而来的雪旺细胞。移植后10天，在移植物中部观察到的增殖雪旺细胞已开始对轴突进行分类。在切碎神经的移植物中，雪旺细胞破碎的基膜重新排列成更粗的束状或小的基膜聚集物。再生轴突无一例外地附着在这种修饰基膜的一侧。胶原纤维与另一侧接触，表明这些修饰基膜在细胞附着方面具有与支架普通基膜相同的极性。（摘要截短于400字）
