Nousek-Goebl N A, Press M F
Brain Res. 1986 Nov;395(1):67-73. doi: 10.1016/s0006-8993(86)80009-9.
Intraparenchymal postnatal development of the CNS vasculature proceeds by a process of vascular 'budding' or 'sprouting' from existing endothelial cells and elongation of these immature vessels. A modified Golgi technique combined with gold toning and deimpregnation was used to identify and characterize sprouting endothelial cells in the neonatal rat cerebellar cortex with light and electron microscopy. Sprouting endothelial cells were the terminal endothelial cells of immature, developing blood vessels and they had characteristic morphologic features. The most distinctive feature was an array of tentacular processes (0.1-0.2 micron in diameter and ca. 20 microns in length) which radiated from the apex of these cells in the presumed direction of vessel growth. Cytoskeletal microtubules and microfilaments were the characteristic organelles of these tentacles. Sprouting endothelial cells were thin (1-3 microns in diameter). lacked a vessel lumen and basement membrane, had abundant cytoplasmic organelles and a prominent nucleus and were closely associated with the subterminal endothelial cell without interendothelial gaps. The developing blood vessels had a blood-brain barrier which excluded intravenously injected colloidal carbon from the neuropil.
中枢神经系统血管系统在脑实质内的产后发育是通过血管从现有内皮细胞“出芽”或“发芽”以及这些未成熟血管伸长的过程进行的。采用改良的高尔基技术结合金染色和脱浸法，通过光学显微镜和电子显微镜鉴定并表征新生大鼠小脑皮质中发芽的内皮细胞。发芽的内皮细胞是未成熟发育血管的终末内皮细胞，具有特征性形态学特征。最显著的特征是一系列触手状突起（直径0.1 - 0.2微米，长度约20微米），从这些细胞的顶端沿假定的血管生长方向放射状伸出。细胞骨架微管和微丝是这些触手的特征性细胞器。发芽的内皮细胞很薄（直径1 - 3微米），没有血管腔和基底膜，有丰富的细胞质细胞器和一个突出的细胞核，并且与亚终末内皮细胞紧密相连，内皮细胞之间没有间隙。发育中的血管具有血脑屏障，可将静脉注射的胶体碳排除在神经毡之外。
