妊娠和子痫前期中组织蛋白酶 C 和组织蛋白酶 D 的表达及意义。
Expression and significance of cathepsin C and cathepsin D during pregnancy and Preeclampsia.
机构信息
Department of Obstetrics and Gynecology, Clinical Medical College, Yangzhou University, 225001, Yangzhou, Jiangsu, China.
Graduate School of Dalian Medical University, Dalian, China.
出版信息
Reprod Biol Endocrinol. 2023 Oct 4;21(1):92. doi: 10.1186/s12958-023-01138-x.
BACKGROUND
Cathepsin C (Cat C) is involved in the inflammatory-immune system and can be degraded by cathepsin D (Cat D). Preeclampsia (PE) and the inflammation-immunity relationship is currently a hot research topic, but there are still few studies. The aim was to investigate the expression and significance of Cat C and D in the serum of nonpregnant women, patients in various stages of pregnancy and patients with PE, and in the placenta of patients with normal pregnancy and PE.
METHODS
Sixty young healthy nonpregnant women were selected: 180 normal pregnant women, including 60 each in the first, second, and third trimesters, and 100 women with PE, including 39 women with severe preeclampsia. The levels of Cat C and D in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the expression levels of Cat C and D in placentas were detected by immunohistochemistry (IHC).
RESULTS
The serum of Cat C in the first trimester was significantly lower than that in the nonpregnant group (P < 0.001), whereas Cat D was significantly higher than that in the nonpregnant group (P < 0.01). The levels of Cat C and D in the second trimester and third trimester were significantly higher than those in the first trimester (P < 0.05), but there was no significant difference in Cat C and D between the second trimester and third trimester. The levels of Cat C in the serum and placentas of patients with PE were significantly higher than those in the third trimester (P < 0.001) and positively correlated with the severity of PE (P < 0.001), whereas the levels of Cat D in the serum and placentas of patients with PE were significantly lower than those in the third trimester (P < 0.001) and negatively correlated with the severity of PE (P < 0.001). Age, primigravida proportion, and body mass index were significantly higher in the PE group than in the control group (P < 0.05), which were high-risk factors for PE.
CONCLUSIONS
Cat C and D are associated with the maintenance of normal pregnancy. In patients with preeclampsia, a significant increase in Cat C and a significant decrease in Cat D levels may lead to the occurrence and development of preeclampsia.
背景
组织蛋白酶 C（Cat C）参与炎症-免疫系统，可被组织蛋白酶 D（Cat D）降解。子痫前期（PE）和炎症-免疫关系是目前的热门研究课题，但相关研究仍较少。本研究旨在探讨非孕妇、不同妊娠阶段孕妇及 PE 患者血清及正常妊娠和 PE 患者胎盘组织中 Cat C 和 D 的表达及意义。
方法
选取 60 名年轻健康的非孕妇：180 名正常孕妇，其中第 1、2、3 个孕期各 60 名，100 名 PE 患者，其中 39 名重度子痫前期患者。采用酶联免疫吸附试验（ELISA）检测血清中 Cat C 和 D 的水平，免疫组化（IHC）检测胎盘组织中 Cat C 和 D 的表达水平。
结果
第 1 孕期血清 Cat C 明显低于非孕妇组（P<0.001），Cat D 明显高于非孕妇组（P<0.01）。第 2、3 孕期血清 Cat C 和 D 水平明显高于第 1 孕期（P<0.05），但第 2、3 孕期血清 Cat C 和 D 水平无明显差异。PE 患者血清和胎盘组织中 Cat C 水平明显高于第 3 孕期（P<0.001），与 PE 严重程度呈正相关（P<0.001），而血清和胎盘组织中 Cat D 水平明显低于第 3 孕期（P<0.001），与 PE 严重程度呈负相关（P<0.001）。PE 组年龄、初产妇比例、体质量指数明显高于对照组（P<0.05），均为 PE 的高危因素。
结论
Cat C 和 D 与维持正常妊娠有关。在子痫前期患者中，Cat C 明显增加，Cat D 水平明显降低，可能导致子痫前期的发生发展。
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