Institute for Computer Science, Heinrich Heine University , Düsseldorf, Germany.
Department of Biology, Heinrich Heine University , Düsseldorf, Germany.
mSystems. 2023 Oct 26;8(5):e0076023. doi: 10.1128/msystems.00760-23. Epub 2023 Oct 5.
Protein translation is the most expensive cellular process in fast-growing bacteria, and efficient proteome usage should thus be under strong natural selection. However, recent studies show that a considerable part of the proteome is unneeded for instantaneous cell growth in . We still lack a systematic understanding of how this excess proteome is distributed across different pathways as a function of the growth conditions. We estimated the minimal required proteome across growth conditions in and compared the predictions with experimental data. We found that the proteome allocated to the most expensive internal pathways, including translation and the synthesis of amino acids and cofactors, is near the minimally required levels. In contrast, transporters and central carbon metabolism show much higher proteome levels than the predicted minimal abundance. Our analyses show that the proteome fraction unneeded for instantaneous cell growth decreases along the nutrient flow in .
蛋白质翻译是快速生长细菌中最昂贵的细胞过程，因此有效的蛋白质组使用应该受到强烈的自然选择。然而，最近的研究表明，在 中，相当一部分蛋白质组对于即时的细胞生长是不必要的。我们仍然缺乏系统的理解，即这种多余的蛋白质组如何作为生长条件的函数分布在不同的途径中。我们在 中估计了不同生长条件下所需的最小蛋白质组，并将预测结果与实验数据进行了比较。我们发现，分配给最昂贵的内部途径的蛋白质组，包括翻译以及氨基酸和辅因子的合成，接近最小所需水平。相比之下，转运蛋白和中心碳代谢的蛋白质组水平远高于预测的最小丰度。我们的分析表明，对于即时细胞生长不必要的蛋白质组分数沿着营养物质流减少。
