Cobleigh M A, Kennedy J L, Hill J H, Lindholm K M, Langenberg P W, Applebaum E L
Cancer Res. 1986 Dec;46(12 Pt 1):6364-8.
Growth in culture of squamous head and neck cancer is hampered by microbial contamination, low plating efficiency, and cellular heterogeneity within tumors. Furthermore, clumps of cells must be removed if plating efficiency is to be accurately determined. Isokinetic velocity sedimentation was applied to 44 primary tumor specimens in an effort to minimize these problems. Seven fractions were evaluated for cell number, clump number, cell viability, clonogenic growth, plating efficiency, and microbial overgrowth. Unseparated specimens were simultaneously cultured. Microbial growth was significantly associated with the highest gradient fraction. Clumps were significantly associated with the lowest gradient fraction. Colony formation was significantly associated with middle gradient isokinetic velocity sedimentation, although seven specimens grew only when fractionated, suggesting the possibility of inhibitor cells within the tumor specimen.
头颈部鳞状细胞癌培养过程中的生长受到微生物污染、低接种效率以及肿瘤内细胞异质性的阻碍。此外，若要准确测定接种效率，必须去除细胞团块。为尽量减少这些问题，对44个原发性肿瘤标本采用了等速沉降法。对七个组分进行了细胞数量、团块数量、细胞活力、克隆生长、接种效率和微生物过度生长的评估。同时培养未分离的标本。微生物生长与最高梯度组分显著相关。团块与最低梯度组分显著相关。集落形成与中等梯度等速沉降显著相关，尽管有七个标本仅在分级后生长，这表明肿瘤标本中可能存在抑制细胞。
