Cobleigh M A, Gallagher P A, Hill J H, Applebaum E L, McGuire W P
Am J Pathol. 1984 Jun;115(3):397-402.
This study was undertaken to define the cloning efficiency of squamous head and neck (H/N) cancer in soft agar. Twenty squamous cell carcinomas of H/N origin were mechanically dissociated and cultured in the human tumor clonogenic assay ( HTSCA ). No growth was observed. Nine ascites specimens from separate patients with ovarian cancer were cultured during the same time period, and six grew, all with more than 30 colonies per plate. Thirty-one additional H/N specimens were enzymatically dissociated and cultured in the HTSCA . Again, no growth was observed. Sixteen of these enzymatically dissociated specimens were simultaneously cultured in identical media without agar. Five specimens grew. We conclude that squamous carcinoma of H/N requires anchorage and fibroblast support for successful growth in culture. Suspension in semisolid media is less effective than liquid tissue culture systems for in vitro growth of this tumor type.
本研究旨在确定头颈部鳞状细胞癌在软琼脂中的克隆效率。将20例头颈部来源的鳞状细胞癌进行机械解离，并在人肿瘤克隆形成试验（HTSCA）中培养。未观察到生长。在同一时期培养了来自9例卵巢癌患者的腹水标本，其中6例生长，每平板均有30多个克隆。另外31例头颈部标本进行酶解并在HTSCA中培养。同样，未观察到生长。其中16例酶解标本同时在不含琼脂的相同培养基中培养。5例标本生长。我们得出结论，头颈部鳞状细胞癌在培养中成功生长需要贴壁和成纤维细胞支持。对于这种肿瘤类型的体外生长，半固体培养基中的悬浮培养不如液体组织培养系统有效。
