DryProTech Lab. and BoRN Research Lab, Chemical Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382055, Gujarat, India.
ACS Appl Bio Mater. 2023 Oct 16;6(10):4111-4126. doi: 10.1021/acsabm.3c00101. Epub 2023 Oct 5.
Wound healing using an alginate-based biopolymeric film is one of the most preferred treatments. However, these films lack mechanical strength (elasticity and tensile strength), show higher initial burst release, and exhibit high vapor permeability. The present study reports the development of nanosheets derived from titanium diboride (10 nm) (NTB)-incorporated biopolymeric films (0.025, 0.05, and 0.1% w/v) using sodium alginate (SA) and carboxymethyl cellulose (CMC) to overcome the shortfalls. The surface properties of the film, nanosheet distribution within the film, and possible interactions with the film are explored by using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), Fourier transform infrared (FTIR), and X-ray diffraction (XRD). These analyses confirm that nanosheets are uniformly distributed in the film and introduce unevenness on the film's surface. The tensile strength of the nanosheet-incorporated film (0.1% NTB film) using UTM is found to be 24.30 MPa (six times higher compared to the blank film), equivalent to human skin. The water vapor transmission rate of the film is also found to be in the desired range (i.e., 2000-2500 g/m day). The biocompatibility of the NTB film is confirmed by the MTT assay test using NIH/3T3 cells and HEK 293 cells. Furthermore, the scratch assay shows that the developed films promote cell migration and proliferation. The antibacterial activity of the film is also demonstrated using a model drug, tetracycline hydrochloride (TCl). Besides, the film exhibits the sustained release of TCl and follows the Korsmeyer-Peppas model for drug release. Overall, the 0.1% w/v NTB film is easy to fabricate, biocompatible and shows superior mechanical properties.
使用基于海藻酸钠的生物聚合膜进行伤口愈合是最受欢迎的治疗方法之一。然而，这些膜缺乏机械强度（弹性和拉伸强度），表现出较高的初始突释，并且具有较高的蒸汽透过率。本研究报告了使用海藻酸钠（SA）和羧甲基纤维素（CMC）制备的纳米片衍生自二硼化钛（10nm）（NTB）的生物聚合膜（0.025、0.05 和 0.1%w/v）的开发，以克服这些缺点。通过扫描电子显微镜（SEM）、能量色散光谱（EDS）、傅里叶变换红外（FTIR）和 X 射线衍射（XRD）研究了膜的表面性质、纳米片在膜内的分布以及与膜的可能相互作用。这些分析证实纳米片均匀分布在膜中，并在膜表面引入了不平整。通过 UTM 对纳米片掺入膜（0.1%NTB 膜）的拉伸强度进行了研究，发现其拉伸强度为 24.30MPa（比空白膜高六倍），相当于人类皮肤。还发现膜的水蒸气透过率也在所需范围内（即 2000-2500g/m2/day）。通过 NIH/3T3 细胞和 HEK 293 细胞的 MTT 测定试验证实了 NTB 膜的生物相容性。此外，划痕试验表明，所开发的膜促进了细胞迁移和增殖。还使用模型药物盐酸四环素（TCl）证明了膜的抗菌活性。此外，该膜还表现出 TCl 的持续释放，并遵循药物释放的 Korsmeyer-Peppas 模型。总体而言，0.1%w/v NTB 膜易于制造，具有生物相容性，并且具有优异的机械性能。
