DryProTech Lab, Chemical Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar, Gujarat 382355, India.
School of Pharmacy, National Forensic Sciences University, Gandhinagar, Gujarat 382007, India.
ACS Appl Mater Interfaces. 2024 Jun 19;16(24):30819-30832. doi: 10.1021/acsami.4c04257. Epub 2024 Jun 7.
Sodium alginate (SA) biopolymeric films have various limitations such as poor mechanical properties, high vapor permeability, lack of antibacterial activity, excessive burst release, and weak cell adhesion. To overcome these limitations, a strategy involving the integration of nanofillers into an SA film matrix is explored. In this context, a cost-effective iron-containing carbon nano biocomposite (FeCNB) nanofiller is developed using a solvent-free technique. This nanocomposite is successfully incorporated into the alginate film matrix at varying concentrations (0.05, 0.1, and 0.15%) aimed at enhancing its physicochemical and biological properties for biomedical applications. Characterization through FESEM and BET analyses confirms the porous nature of the FeCNB. EDX shows the FeCNB's uniform distribution upon its integration into the film matrix, albeit without strong chemical interaction with SA. Instead, hydrogen bonding interactions become apparent in the FTIR spectra. By incorporating the FeCNB, the mechanical attributes of the films are improved and the water vapor permeability approaches the desired range (2000-2500 g/mday). The film's swelling ratio reduction contributes to a decrease in water permeability. The antibacterial activity and sustained release property of the FeCNB-incorporated film are established using tetracycline hydrochloride (TCl), a model drug. The drug release profile resembled Korsmeyer-Peppas's release pattern. assessments via the MTT assay and scratch assay on NIH-3T3 cells reveal that FeCNB has no adverse effects on the biocompatibility of alginate films. The cell proliferation and adhesion to the SA film are significantly enhanced after infusion of the FeCNB. The study performed on the rat model demonstrates improved wound healing by FeCNB-impregnated films. Based on the comprehensive findings, the proposed FeCNB-incorporated alginate films prove to be a promising candidate for robust skin repair.
海藻酸钠（SA）生物聚合物膜具有各种局限性，例如机械性能差、蒸汽透过率高、缺乏抗菌活性、突释过多以及细胞黏附力弱。为了克服这些局限性，探索了将纳米填料整合到 SA 膜基质中的策略。在这种情况下，使用无溶剂技术开发了一种具有成本效益的含铁碳纳米生物复合材料（FeCNB）纳米填料。成功地将这种纳米复合材料以不同浓度（0.05、0.1 和 0.15%）掺入藻酸盐膜基质中，旨在增强其物理化学和生物特性，以用于生物医学应用。FESEM 和 BET 分析的特性表明 FeCNB 具有多孔性。EDX 表明 FeCNB 在整合到膜基质中时具有均匀的分布，尽管与 SA 没有强烈的化学相互作用。相反，在 FTIR 光谱中出现氢键相互作用。通过掺入 FeCNB，可以改善膜的机械性能，并使水蒸气透过率接近所需范围（2000-2500 g/mday）。膜的溶胀比降低有助于降低水渗透率。使用盐酸四环素（TCl）作为模型药物，建立了掺入 FeCNB 的膜的抗菌活性和缓释性能。药物释放曲线类似于 Korsmeyer-Peppas 的释放模式。通过 MTT 测定和 NIH-3T3 细胞划痕试验评估，发现 FeCNB 对藻酸盐膜的生物相容性没有不良影响。FeCNB 注入后，SA 膜的细胞增殖和黏附显著增强。在大鼠模型上进行的研究表明，FeCNB 浸渍膜可改善伤口愈合。基于综合研究结果，提出的掺入 FeCNB 的藻酸盐膜被证明是一种有前途的强力皮肤修复候选物。
