转录组分析揭示了长非编码 RNA 参与遮荫诱导生长促进。

Transcriptome Analysis Reveals Long Non-Coding RNAs Involved in Shade-Induced Growth Promotion in .

机构信息

Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui, China.

出版信息

Front Biosci (Landmark Ed). 2023 Sep 14;28(9):202. doi: 10.31083/j.fbl2809202.

DOI:10.31083/j.fbl2809202

PMID:37796682

Abstract

BACKGROUND

High temperature and drought environments are important limiting factors for growth, whereas shading can promote growth by relieving these stresses. However, the mechanism of growth promotion by shading in . is unknown. Long non-coding RNAs (lncRNAs) play important roles in the plant's growth and environmental response, but few analyses of lncRNAs in . have been reported.

METHODS

We performed lncRNAs analysis of . in response to shading using RNA-seq data from our previous studies. A total of 13,927 lncRNAs were identified, and 145 differentially expressed lncRNAs (DELs) were obtained from the comparisons of 5 days shade (D5S) vs. 5 days of natural light (D5CK), 20 days of shade (D20S) vs. 20 days of natural light (D20CK), D20S vs. D5S, and D20CK vs. D5CK. Of these, 119 DELs (82.07%) were generated from the D20S vs. D20CK comparison.

RESULTS

Gene ontology (GO) analysis indicated that the reactive oxygen (ROS) metabolism and programmed cell death (PCD) processes might regulate shade-induced growth promotion. The "signal transduction" and "environmental adaptation" in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used for lncRNA-mRNA regulatory network construction and showed that the lncRNAs might mediate . growth by regulating ROS accumulation and light signals.

CONCLUSIONS

This study explores lncRNAs' functions and regulatory mechanisms related to . growth and lays a foundation for further research on . .

摘要

背景

高温和干旱环境是生长的重要限制因素，而遮荫可以通过缓解这些胁迫来促进生长。然而，遮荫促进生长的机制在中尚不清楚。长链非编码 RNA（lncRNA）在植物的生长和环境响应中发挥着重要作用，但对中 lncRNA 的分析报道较少。

方法

我们利用先前研究的 RNA-seq 数据，对中遮荫响应的 lncRNA 进行了分析。共鉴定出 13927 个 lncRNA，从 5 天遮荫（D5S）与 5 天自然光（D5CK）、20 天遮荫（D20S）与 20 天自然光（D20CK）、D20S 与 D5S、D20CK 与 D5CK 的比较中获得了 145 个差异表达的 lncRNA（DEL）。其中，119 个 DEL（82.07%）来自 D20S 与 D20CK 的比较。