College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211800, Jiangsu, China.
Bioresource Institute for Healthy Utilization, Zunyi Medical University, Zunyi, 563000, Guizhou, China.
Sci Rep. 2020 Mar 12;10(1):4614. doi: 10.1038/s41598-020-61512-2.
Pinellia (Pinellia ternata (Thunb.) Breit.), as important medicinal plant, has been used to treat various ailments for a long time. The sixteen ploid plant (2n = 16 * 13 = 208) Pinellia T2Plus line was obtained from an octoploid (2n = 8 * 13 = 104) T2 line by chromosome-doubling technique. Compared with T2 line, the content of various medicinal components (polysaccharide, guanosine, adenosine and ephedrine) was increased in T2Plus line. In this study, the transcriptome of T2 line and T2Plus line were characterized by RNA sequencing (RNA-seq) technology. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis on differential expressed unigenes (DEGs) revealed that multiple metabolic pathway were enriched significantly, such as 'Starch and sucrose metabolism', 'Purine metabolism', 'Photosynthesis' and six transcription factors (MYB, WRKY, bHLH, lateral organ boundaries domain (LBD), homeodomain-zipper (HD-ZIP) and Ethylene-responsive factor (ERF)) play a key role in difference of transcriptome between T2 line and T2Plus line. These metabolic pathways and transcription factors may play an important role in the difference of medicinal components and epigenetic features between these two Pinellia cultivars. This conclusion provides a robust theoretical basis for the mechanism of the formation of medicinal ingredients in Pinellia cultivars.
半夏（Pinellia ternata (Thunb.) Breit.）作为一种重要的药用植物，长期以来一直被用于治疗各种疾病。十六倍体植物（2n=1613=208）半夏 T2Plus 系是通过染色体加倍技术从八倍体（2n=813=104）T2 系获得的。与 T2 系相比，T2Plus 系中各种药用成分（多糖、鸟苷、腺苷和麻黄碱）的含量增加。在这项研究中，通过 RNA 测序（RNA-seq）技术对 T2 系和 T2Plus 系的转录组进行了表征。差异表达基因（DEGs）的基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径富集分析表明，多个代谢途径显著富集，如“淀粉和蔗糖代谢”、“嘌呤代谢”、“光合作用”和六个转录因子（MYB、WRKY、bHLH、侧生器官边界域（LBD）、同源域-拉链（HD-ZIP）和乙烯响应因子（ERF））在 T2 系和 T2Plus 系转录组差异中发挥关键作用。这些代谢途径和转录因子可能在这两个半夏品种的药用成分和表观遗传特征差异中发挥重要作用。这一结论为半夏品种药用成分形成的机制提供了坚实的理论基础。
