College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Hangzhou 310018, China.
Genes (Basel). 2023 Aug 29;14(9):1727. doi: 10.3390/genes14091727.
(Thunb.) Breit (abbreviated as ) is a plant with an important medicinal value whose yield is restricted by many factors, such as low reproductive efficiency and continuous cropping obstacles. As an essential breeding material for growth and production, the bulbils have significant advantages such as a high survival rate and short breeding cycles. However, the location effect, influencing factors, and molecular mechanism of bulbil occurrence and formation have not been fully explored. In this study, exogenously applied phytohormones were used to induce in vitro petiole of to produce bulbil structure. Transcriptome sequencing of mRNA and miRNA were performed in the induced petiole (TCp) and the induced bulbil (TCb). Gene Ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed for the identification of key genes and pathways involved in bulbil development. A total of 58,019 differentially expressed genes (DEGs) were identified. The GO and KEGG analysis indicated that DEGs were mainly enriched in plant hormone signal transduction and the starch and sucrose metabolism pathway. The expression profiles of miR167a, miR171a, and miR156a during bulbil induction were verified by qRT-PCR, indicating that these three miRNAs and their target genes may be involved in the process of bulbil induction and play an important role. However, further molecular biological experiments are required to confirm the functions of the identified bulbil development-related miRNAs and targets.
（Thunb.） Breit（简称）是一种具有重要药用价值的植物，其产量受到许多因素的限制，如繁殖效率低和连作障碍。作为 生长和生产的重要繁殖材料，鳞茎具有成活率高、繁殖周期短等显著优势。然而，鳞茎发生和形成的位置效应、影响因素和分子机制尚未得到充分探索。本研究采用外源植物激素诱导 离体叶柄产生鳞茎结构。对诱导的叶柄（TCp）和诱导的鳞茎（TCb）进行 mRNA 和 miRNA 的转录组测序。对关键基因和途径进行基因本体论（GO）术语富集和京都基因与基因组百科全书（KEGG）途径分析，以鉴定参与鳞茎发育的关键基因和途径。共鉴定出 58019 个差异表达基因（DEGs）。GO 和 KEGG 分析表明，DEGs 主要富集在植物激素信号转导和淀粉和蔗糖代谢途径中。通过 qRT-PCR 验证了 miR167a、miR171a 和 miR156a 在鳞茎诱导过程中的表达谱，表明这三个 miRNA 及其靶基因可能参与鳞茎诱导过程，并发挥重要作用。然而，需要进一步的分子生物学实验来验证鉴定出的与鳞茎发育相关的 miRNA 和靶基因的功能。
