Department of Pathology, Faculty of Veterinary Medicine, University of Aydın Adnan Menderes, 09016-Isikli, Aydin, Turkiye.
Department of Virology, Faculty of Veterinary Medicine, University of Ankara, Diskapi, 06110 Ankara, Turkiye.
J Comp Pathol. 2023 Oct;206:36-43. doi: 10.1016/j.jcpa.2023.08.008. Epub 2023 Oct 3.
Acute demyelinating leucoencephalomyelitis was the most conspicuous microscopic change in the brain and spinal cord of kids infected with caprine arthritis encephalitis virus (CAEV). TUNEL positivity and labelling of anti-bax and anti-caspases-3, -8 and -9 were found in a distinct population of glial cells, mainly at the edges of the demyelinated plaques and perivascular areas and, to a lesser extent, in neurons. Double labelling revealed that most of these apoptotic cells in the demyelinated plaques were astrocytes and a few were oligodendroglia. In contrast, expression of bcl-2, an anti-apoptotic protein, was found mainly in neurons of the brainstem and cerebellum and motor neurons of the spinal cord, but was restricted in glial cells. These results suggest that apoptosis plays an important role in the pathogenesis of CAE demyelinating encephalitis.
急性脱髓鞘性脑脊髓炎是感染山羊关节炎脑炎病毒（CAEV）的儿童脑部和脊髓最显著的微观变化。TUNEL 阳性和抗 bax 及抗 caspase-3、-8 和 -9 的标记在明显的神经胶质细胞群中发现，主要在脱髓鞘斑块的边缘和血管周围区域，在神经元中则较少。双标记显示，脱髓鞘斑块中的大多数凋亡细胞是星形胶质细胞，少数是少突胶质细胞。相比之下，抗凋亡蛋白 bcl-2 的表达主要在脑干和小脑的神经元以及脊髓的运动神经元中发现，但在神经胶质细胞中受到限制。这些结果表明，细胞凋亡在 CAE 脱髓鞘性脑炎的发病机制中起重要作用。
