School of Life Sciences, Nanchang University, Nanchang 330031, Jiangxi, PR China.
School of Life Sciences, Nanchang University, Nanchang 330031, Jiangxi, PR China.
Gene. 2024 Jan 20;892:147869. doi: 10.1016/j.gene.2023.147869. Epub 2023 Oct 4.
NF-Y, a critical transcription factor, binds to the CCAAT-box in target gene promoters, playing a pivotal role in plant development and abiotic stress response. OsNF-YC5, encodes a putative subunit of the NF-Y transcription factor in rice, had an undetermined function. Our research revealed that OsNF-YC5 is induced by high salinity and exogenous abscisic acid (ABA). Subcellular localization studies showed that OsNF-YC5 is nuclear- and cytoplasm-localized. Using CRISPR-Cas9 to disrupt OsNF-YC5, we observed significantly enhanced rice salinity tolerance and ABA-hypersensitivity. Compared to the wild-type, osnf-yc5 mutants exhibited reduced HO and malondialdehyde (MDA) levels, increased catalase (CAT) activity, and elevated OsCATA transcripts under salt stress. Moreover, ABA-dependent (OsABI2 and OsLEA3) and ABA-independent (OsDREB1A, OsDREB1B, and OsDREB2A) marker genes were upregulated in mutant lines in response to salinity. These results indicate that disrupting OsNF-YC5 enhances rice salinity tolerance, potentially by boosting CAT enzyme activity and modulating gene expression in both ABA-dependent and ABA-independent pathways. Therefore, this study provides a valuable theoretical foundation and genetic resources for developing novel salt-tolerant rice varieties.
NF-Y 是一种关键的转录因子，能与靶基因启动子中的 CCAAT 盒结合，在植物发育和非生物胁迫响应中起着关键作用。OsNF-YC5 是水稻中 NF-Y 转录因子的一个假定亚基，其功能尚未确定。我们的研究表明，OsNF-YC5 受高盐和外源脱落酸（ABA）诱导。亚细胞定位研究表明，OsNF-YC5 定位于核和细胞质。通过 CRISPR-Cas9 技术破坏 OsNF-YC5，我们观察到水稻的耐盐性和 ABA 敏感性显著增强。与野生型相比，osnf-yc5 突变体在盐胁迫下 HO 和丙二醛（MDA）水平降低，过氧化氢酶（CAT）活性增加，OsCATA 转录物水平升高。此外，ABA 依赖型（OsABI2 和 OsLEA3）和 ABA 非依赖型（OsDREB1A、OsDREB1B 和 OsDREB2A）标记基因在突变体中对盐胁迫的响应均上调。这些结果表明，破坏 OsNF-YC5 可增强水稻的耐盐性，可能是通过提高 CAT 酶活性并调节 ABA 依赖型和 ABA 非依赖型途径中的基因表达。因此，本研究为培育新型耐盐水稻品种提供了有价值的理论基础和遗传资源。
