利用经代谢工程改造的深绿链霉菌，以浒苔高效生产伊拉霉素。
Efficient ilamycins production utilizing Enteromorpha prolifera by metabolically engineered Streptomyces atratus.
作者信息
Jiang Yu-Xi, Zheng Gao-Fan, Chen Long-Chao, Yang Na, Xin Xiu-Juan, Ma Jun-Ying, Ju Jian-Hua, Wu Hui, Zhao Ming, Wang Ruida, An Fa-Liang
机构信息
State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 528225, China.
出版信息
Biotechnol Biofuels Bioprod. 2023 Oct 5;16(1):151. doi: 10.1186/s13068-023-02398-w.
With the invasion of green tides and the increase of urban green areas worldwide, multimillion tons of Enteromorpha need to be reutilized. In this study, Enteromorpha prolifera powder is considered a promising biomass resource for the production of commercial chemical products production. Ilamycins, novel cyclic heptapeptides with significant anti-TB activities, are isolated from Streptomyces atratus SCSIO ZH16, a deep-sea-derived strain. Using EP powder as a nitrogen source, the production of ilamycins reached 709.97 mg/L through optimization of the nitrogen source using the engineered strain S. atratus SCSIO ZH16 ΔR. After mutant strain constructions and tests, strain S. atratus SCSIO ZH16 ΔR::bldD EP powder achieved a higher production titer of ilamycins. Furthermore, the production titer of ilamycins and ilamycin E reached 1561.77 mg/L and 745.44 mg/L, respectively, in a 5 L bioreactor. This study suggests that E. prolifera is a promising and eco-friendly nitrogen source for the production of ilamycins.
随着绿潮的侵袭以及全球城市绿地面积的增加，数以百万吨计的浒苔需要得到再利用。在本研究中，浒苔粉被认为是用于生产商业化学产品的一种有前景的生物质资源。伊拉霉素是从深海来源的菌株黑暗链霉菌SCSIO ZH16中分离得到的具有显著抗结核活性的新型环状七肽。以浒苔粉作为氮源，通过对工程菌株黑暗链霉菌SCSIO ZH16 ΔR的氮源进行优化，伊拉霉素的产量达到了709.97 mg/L。经过突变菌株构建和测试，黑暗链霉菌SCSIO ZH16 ΔR::bldD浒苔粉实现了更高的伊拉霉素产量。此外，在5 L生物反应器中，伊拉霉素和伊拉霉素E的产量分别达到了1561.77 mg/L和745.44 mg/L。本研究表明，浒苔是用于生产伊拉霉素的一种有前景且环保的氮源。
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