Liu Quangang, Wen Jiaxing, Wang Shipeng, Chen Jianhua, Sun Yongqiang, Liu Qingbai, Li Xi, Dong Shengjun
College of Forestry, Shenyang Agricultural University, Shenyang, China.
Key Laboratory for Silviculture of Liaoning, Shenyang Agricultural University, Shenyang, China.
Front Plant Sci. 2023 Sep 20;14:1267107. doi: 10.3389/fpls.2023.1267107. eCollection 2023.
The basic helix-loop-helix (bHLH) family is one of the most well-known transcription factor families in plants, and it regulates growth, development, and abiotic stress responses. However, systematic analyses of the bHLH gene family in have not been reported to date. In this study, 104 were identified and classified into 23 subfamilies that were unevenly distributed on eight chromosomes. Nineteen pairs of segmental replication genes and ten pairs of tandem replication genes were identified, and all duplicated gene pairs were under purifying selection. of the same subfamily usually share similar motif compositions and exon-intron structures. contain multiple stress-responsive elements. exhibit functional diversity by interacting and coordinating with other members. Twenty showed varying degrees of expression. Eleven genes up-regulated and nine genes down-regulated in -4°C. The majority of were highly expressed in the roots and pistils. Transient transfection experiments demonstrated that transgenic plants with overexpressed have better cold tolerance. In conclusion, the results of this study have significant implications for future research on the involvement of bHLH genes in the development and stress responses of .
基本螺旋-环-螺旋（bHLH）家族是植物中最著名的转录因子家族之一，它调控生长、发育和非生物胁迫响应。然而，迄今为止尚未见关于[具体物种]中bHLH基因家族的系统分析报道。在本研究中，共鉴定出104个[具体基因]，并将其分为23个亚家族，这些亚家族在8条染色体上分布不均。鉴定出19对片段重复基因和10对串联重复基因，且所有重复基因对均受到纯化选择。同一亚家族的[具体基因]通常具有相似的基序组成和外显子-内含子结构。[具体基因]含有多个胁迫响应元件。[具体基因]通过与其他成员相互作用和协调表现出功能多样性。20个[具体基因]表现出不同程度的表达。在-4°C条件下，11个基因上调表达，9个基因下调表达。大多数[具体基因]在根和雌蕊中高表达。瞬时转染实验表明，过表达[具体基因]的转基因植物具有更好的耐寒性。总之，本研究结果对未来研究bHLH基因参与[具体物种]的发育和胁迫响应具有重要意义。
