Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006086.
A facultative anaerobic and Gram-negative strain, designated RP14, was isolated from the fruit of fermented for 60 days at 25°C. Strain RP14 showed 98.0 % 16S rRNA similarity to IFO 15243, but in the phylogenetic tree, NIBRBAC000500504 was its closest neighbour. The average nucleotide identity and digital DNA-DNA hybridization values between strain RP14 and 15 genomes of type strains of , were 73.8-74.4% and 16.4-20.2 %, respectively, which were lower than the recommended thresholds for species delineation. The strain grew at 25-32°C (optimum, 28°C), at pH 7.0-12.0 (optimum, pH 9.0) and with 0-2% NaCl (optimum, 0 %; w/v). Cells of strain RP14 were catalase-positive, oxidase-negative, rod-shaped and formed yellow-coloured colonies. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major fatty acid were C, C cyclo 8 and summed feature 8 (C 7 and/or C 6). The DNA G+C content was 62.8 mol%. Based on polyphasic evidence, we propose sp. nov as a novel species within the genus The type strain is RP14 (=KACC 22720=TBRC 16341).
一株兼性厌氧革兰氏阴性菌株，命名为 RP14，从发酵 60 天的水果中分离得到，发酵温度为 25°C。菌株 RP14 与 IFO 15243 的 16S rRNA 相似性为 98.0%，但在系统发育树中，NIBRBAC000500504 是其最接近的近缘种。菌株 RP14 与 15 株模式菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 73.8-74.4%和 16.4-20.2%，均低于种界定的推荐阈值。该菌株在 25-32°C（最佳温度为 28°C）、pH 7.0-12.0（最佳 pH 为 9.0）和 0-2%NaCl（最佳为 0%；w/v）的条件下生长。菌株 RP14 的细胞呈过氧化氢酶阳性、氧化酶阴性、杆状，形成黄色菌落。主要极性脂为磷脂酰乙醇胺、双磷脂酰甘油和磷脂酰甘油。主要脂肪酸为 C 16:0、C 18:1 cyclo 和特征脂肪酸 8（C 7 和/或 C 6）。DNA G+C 含量为 62.8 mol%。基于多相证据，我们建议将 sp. nov. 作为该属中的一个新种。模式菌株为 RP14（=KACC 22720=TBRC 16341）。
