College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 311215, China.
Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Talanta. 2024 Jan 15;267:125258. doi: 10.1016/j.talanta.2023.125258. Epub 2023 Sep 30.
Point-of-care nucleic acid detection is essential for diagnosis and food safety, especially in resource-limited areas. This study reports a gravity-driven and rotation-controlled (GR) chip-coupled lateral flow-based assay (LFA) for point-of-care nucleic acid detection. The sample solution is added to the inlet of the GR chip and flows into the loop-medicated isothermal amplification (LAMP) chamber by the action of gravity. After the LAMP reaction, the GR chip is manually rotated to allow the amplified solution to flow into the LFA chamber for result readout. The GR chip integrates the LAMP reaction and LFA in a fully closed environment, avoiding the aerosol contamination problem. The GR chip's simple and ingenious structure simplifies the fabrication and reduces its cost, making it possible for point-of-care and even home self-testing.
即时检测的核酸检测对于诊断和食品安全至关重要，尤其是在资源有限的地区。本研究报告了一种基于侧向流动的基于重力驱动和旋转控制（GR）芯片的即时检测核酸检测方法。将样品溶液加入到 GR 芯片的入口，在重力的作用下流入环介导等温扩增（LAMP）室。LAMP 反应后，手动旋转 GR 芯片，使扩增溶液流入 LFA 室进行结果读取。GR 芯片将 LAMP 反应和 LFA 集成在一个完全封闭的环境中，避免了气溶胶污染问题。GR 芯片的简单巧妙结构简化了制造过程并降低了成本，使其能够实现即时检测，甚至是家庭自检。
