Lyu Jiang-Nan, Zhang Ling-Xia, Yang Qing-Yu, Huang Na, Wang Zhi-Min, Dai Li-Ping
Henan University of Chinese Medicine Zhengzhou 450046, China Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province, Henan University of Chinese Medicine Zhengzhou 450046, China.
Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province, Henan University of Chinese Medicine Zhengzhou 450046, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2023 Sep;48(18):5024-5031. doi: 10.19540/j.cnki.cjcmm.20230517.202.
Chemical constituents were isolated and purified from ethyl acetate fraction of Arctium lappa leaves by silica gel, ODS, MCI, and Sephadex LH-20 column chromatography. Their structures were identified with multiple spectroscopical methods including NMR, MS, IR, UV, and X-ray diffraction combined with literature data. Twenty compounds(1-20) were identified and their structures were determined as arctanol(1), citroside A(2), melitensin 15-O-β-D-glucoside(3), 11β,13-dihydroonopordopicrin(4), 11β,13-dihydrosalonitenolide(5), 8α-hydroxy-β-eudesmol(6), syringin(7), dihydrosyringin(8), 3,4,3',4'-tetrahydroxy-δ-truxinate(9),(+)-pinoresinol(10), phillygenin(11), syringaresinol(12), kaeperferol(13), quercetin(14), luteolin(15), hyperin(16), 4,5-O-dicaffeoylquinic acid(17), 1H-indole-3-carboxaldehyde(18), benzyl-β-D-glucopyranoside(19), and N-(2'-phenylethyl) isobutyramide(20). Among them, compound 1 is a new norsesquiterpenoid, and compounds 2-5, 7-8, and 18-20 are isolated from this plant for the first time.
通过硅胶柱色谱、ODS柱色谱、MCI柱色谱和葡聚糖凝胶LH - 20柱色谱从牛蒡叶的乙酸乙酯部位分离并纯化化学成分。采用包括核磁共振（NMR）、质谱（MS）、红外光谱（IR）、紫外光谱（UV）和X射线衍射在内的多种光谱方法结合文献数据鉴定其结构。鉴定出20种化合物（1 - 20），其结构确定为牛蒡醇（1）、柠檬苷A（2）、蜂斗菜苷15 - O - β - D - 葡萄糖苷（3）、11β,13 - 二氢苦鬼臼毒素（4）、11β,13 - 二氢异土木香内酯（5）、8α - 羟基 - β - 桉叶醇（6）、紫丁香苷（7）、二氢紫丁香苷（8）、3,4,3',4' - 四羟基 - δ - 吐昔酸（9）、（ + ） - 松脂醇（10）、连翘酯素（11）、紫丁香树脂醇（12）、山柰酚（13）、槲皮素（14）、木犀草素（15）、金丝桃苷（16）、4,5 - O - 二咖啡酰奎宁酸（17）、1H - 吲哚 - 3 - 甲醛（18）、苄基 - β - D - 吡喃葡萄糖苷（19）和N - （2' - 苯乙基）异丁酰胺（20）。其中，化合物1是一种新的降倍半萜类化合物，化合物2 - 5、7 - 8和18 - 20是首次从该植物中分离得到。
