Yang Yong-Cheng, Wei Jiang-Chun, Liu Lu-Qi, Fan San-Peng, Chen Min, Wang An-Hua, Jia Jing-Ming
School of Chinese Materia Medica, Shenyang Pharmaceutical University Shenyang 110016, China.
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Shenyang 110033, China.
Zhongguo Zhong Yao Za Zhi. 2022 Mar;47(6):1582-1586. doi: 10.19540/j.cnki.cjcmm.20211203.201.
This study investigated the chemical components from the leaves and stems of Schisandra chinensis. Three norsesquiterpenoids were isolated from S. chinensis by various column chromatographies(silica gel, Sephadex LH-20, and MCI), reversed-phase medium-pressure preparative, and semi-preparative high-performance liquid chromatography(HPLC). Their structures were identified based on physicochemical properties, mass spectrometry(MS), nuclear magnetic resonance(NMR), ultraviolet(UV), and electro-nic circular dichroism(ECD) as(3R,4R,5R,6S,7E)-3,4,5,6-tetrahydroxy-7-megastigmen-9-one(1),(3S,5R,6R,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(2), and(3S,4R,9R)-3,4,9-trihydroxymegastigman-5-ene(3). Compound 1 was a new compound, and its absolute configuration was determined by ECD. Compounds 2 and 3 were isolated from the Schisandra plant for the first time.
本研究对五味子叶和茎的化学成分进行了研究。通过各种柱色谱法（硅胶、葡聚糖凝胶LH - 20和MCI）、反相中压制备色谱以及半制备高效液相色谱（HPLC）从五味子中分离出三种降倍半萜类化合物。基于其物理化学性质、质谱（MS）、核磁共振（NMR）、紫外光谱（UV）和电子圆二色光谱（ECD）确定了它们的结构，分别为(3R,4R,5R,6S,7E)-3,4,5,6 - 四羟基 - 7 - 巨豆三烯 - 9 - 酮（1）、(3S,5R,6R,7E)-3,5,6 - 三羟基 - 7 - 巨豆三烯 - 9 - 酮（2）和(3S,4R,9R)-3,4,9 - 三羟基巨豆甾 - 5 - 烯（3）。化合物1是一种新化合物，其绝对构型通过ECD确定。化合物2和3首次从五味子属植物中分离得到。
