Corporación Colombiana de Investigación Agropecuaria - Agrosavia. Centro de Investigación La Selva, Kilómetro 7, Vía a Las Palmas, vereda Llano Grande, Rionegro Antioquia, Colombia; French Agricultural Research Centre for International Development (CIRAD), UMR Qualisud, Rionegro (Ant.), Colombia; Joint Research Unit-UMR Qualisud, Univ Montpellier, Univ. d'Avignon, CIRAD, Institut Agro, IRD, Université de La Réunion, Montpellier, France.
Corporación Colombiana de Investigación Agropecuaria - Agrosavia. Centro de Investigación La Selva, Kilómetro 7, Vía a Las Palmas, vereda Llano Grande, Rionegro Antioquia, Colombia.
Food Res Int. 2023 Nov;173(Pt 2):113443. doi: 10.1016/j.foodres.2023.113443. Epub 2023 Sep 11.
The metabolites entering the bloodstream and being excreted in urine as a result of consuming golden berries are currently unidentified. However, these metabolites potentially underlie the health benefits observed in various in vitro, animal, and human models. A nutritional intervention with 18 healthy human volunteers was performed, and urine was collected at baseline and after acute and short-term fruit consumption for 19 days. After UPLC-ESI/QToF-MS analysis, untargeted metabolomics was performed on the urine samples, and from the 50 most discriminant ions (VIP > 2) generated by a validated PLS-DA model (CV-ANOVA = 3.7e-35; R^2Y = 0.86, Q^2Y = 0.62 and no overfitting), 22 compounds were identified with relatively high confidence. The most discriminant metabolites confirmed by DHS/GC-MS analysis of volatiles in urine were sesquiterpenes (CH): 3 stereoisomers, β-vatirenene, β-vetivenene, and β-vetispirene, and 2 isomers, eremophila-1(10),8,11-triene and α-curcumene. Another major urinary biomarker was 4β-hydroxywithanolide E and its phase II derivatives, which were observed in urine for all individual up to 24 h after the fruit was consumed; thus, the bioavailability of this biomarker in humans was demonstrated for the first time. Additionally, the excretion of certain acylcarnitines and hypoxanthine in urine increased after golden berry consumption, which may be associated with a detoxifying effect and may occur because fats were utilized rather than carbohydrates to meet the body's energy needs. The main biomarkers of golden berry consumption are specific to this fruit, confirming its potential for the functional food market.
食用黄金莓后进入血液并随尿液排出的代谢物目前尚未确定。然而，这些代谢物可能是各种体外、动物和人体模型中观察到的健康益处的基础。对 18 名健康志愿者进行了营养干预，在基线时以及急性和短期水果摄入 19 天后收集尿液。在 UPLC-ESI/QToF-MS 分析后，对尿液样本进行非靶向代谢组学分析，从验证的 PLS-DA 模型生成的 50 个最具区分性的离子（VIP > 2）（CV-ANOVA = 3.7e-35；R^2Y = 0.86，Q^2Y = 0.62，无过度拟合）中，鉴定出 22 种具有相对高可信度的化合物。通过 DHS/GC-MS 分析尿液中挥发性物质，对最具区分性的代谢物进行确认，结果表明这些代谢物为倍半萜烯（CH）：3 个立体异构体，β-缬草烯、β-缬草烯和 β-牛膝烯，以及 2 个异构体，依兰-1(10)，8，11-三烯和 α-姜黄烯。另一个主要的尿生物标志物是 4β-羟基乌苏烷醇 E 及其 II 期衍生物，在水果摄入后所有个体的尿液中均可观察到，24 小时内；因此，首次证明了该生物标志物在人体内的生物利用度。此外，黄金莓摄入后尿液中某些酰基辅酶 A 和次黄嘌呤的排泄增加，这可能与解毒作用有关，并且可能是因为脂肪被利用而不是碳水化合物来满足身体的能量需求。黄金莓消费的主要生物标志物是该水果特有的，证实了其在功能性食品市场的潜力。
