Haddad Z H, Kalra V, Verma S
Ann Allergy. 1979 Jun;42(6):368-71.
The sera of 20 atopic patients, 10 with hypersensitivity symptoms upon ingestion of milk and 10 without such symptoms, were tested by a radioimmunoassay for the detection of IgE antibodies to whole bovine milk, betalactoglobulin (BL), enzymatic digests of BL and fractions thereof. While only 4/10 patients had IgE antibodies to undigested BL all 10 patients had IgE antibodies to BL digests. All controls gave negative results. These findings suggest that enzymatic digestion of BL may yield antigenic fractions which elicit IgE antibodies and that these antibodies would be missed if the undigested food protein were to be used solely in testing.
对20名特应性患者的血清进行了检测，其中10名患者在摄入牛奶后出现过敏症状，另外10名患者没有此类症状。采用放射免疫分析法检测这些血清中针对全脂牛奶、β-乳球蛋白（BL）、BL酶解产物及其组分的IgE抗体。虽然10名患者中只有4名对未消化的BL有IgE抗体，但所有10名患者对BL酶解产物都有IgE抗体。所有对照结果均为阴性。这些发现表明，BL的酶解可能产生能引发IgE抗体的抗原性组分，并且如果仅使用未消化的食物蛋白进行检测，这些抗体会被遗漏。
