Barnes R M, Barton P G, Doig J E, Finn R, Harvey M M, Johnson P M
J Clin Lab Immunol. 1983 Dec;12(4):175-8.
Sera from 262 blood donors were tested against wheat gliadin and bovine milk antigens employing an ELISA system. 9.2% of the sera clearly contained antibodies, predominantly IgG but also including IgA and occasional IgM, reactive with either gliadin (11/262) or milk (13/262). There was no overlap between the gliadin and milk groups, and only one of these sera was also IgE-RAST-positive against wheat or milk. Five of 48 sera with raised IgE levels (greater than or equal to 100 iu/ml) were ELISA-positive against either gliadin or milk, a frequency comparable with that overall in this normal adult population. Six of these 48 sera with raised IgE levels were IgE-RAST-positive against wheat or milk, of which only one was ELISA-positive. These results have indicated that nearly 10% of normal adults have serum IgG/A/M antibodies to milk or gliadin, but that this population is separate from individuals (2.3%) who may be IgE-RAST-positive to the same dietary antigens.
采用酶联免疫吸附测定（ELISA）系统，对262名献血者的血清进行了针对小麦醇溶蛋白和牛乳抗原的检测。9.2%的血清明显含有抗体，主要为IgG，但也包括IgA和偶尔的IgM，与醇溶蛋白（11/262）或牛奶（13/262）发生反应。醇溶蛋白组和牛奶组之间没有重叠，并且这些血清中只有一份对小麦或牛奶的IgE-RAST检测呈阳性。48份IgE水平升高（大于或等于100 iu/ml）的血清中有5份对醇溶蛋白或牛奶的ELISA检测呈阳性，这一频率与该正常成年人群的总体频率相当。这48份IgE水平升高的血清中有6份对小麦或牛奶的IgE-RAST检测呈阳性，其中只有一份ELISA检测呈阳性。这些结果表明，近10%的正常成年人血清中存在针对牛奶或醇溶蛋白的IgG/A/M抗体，但该群体与可能对相同饮食抗原有IgE-RAST阳性反应的个体（2.3%）不同。
