孟加拉国出现令人关注的 SARS-CoV-2 变异株 B.1.525（Eta）。

Emergence of SARS-CoV-2 variant of interest B.1.525 (Eta) in Bangladesh.

机构信息

Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh.

National Institute of Laboratory Medicine and Referral Center, Dhaka, Bangladesh.

出版信息

Biologicals. 2023 Nov;84:101714. doi: 10.1016/j.biologicals.2023.101714. Epub 2023 Oct 5.

DOI:10.1016/j.biologicals.2023.101714

PMID:37804694

Abstract

In the present study, we report the complete genome of five Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from Bangladesh harboring mutations at Spike protein (E484K, Q677H, D614G, A67V, Q52R, Y144del, H69del, V70del, F888L) assigned to the B.1.525 lineage (Variant of interest). Mutations are also found in viral structural proteins other than spike region (E_L21F, M_I82F, N_A12G and N_T208I) and other mutations (NSP3_T1189I, NSP6_S106del, NSP6_F108del, NSP6_G107del, NSP12_P323F) from all of five B.1.525 SARS-CoV-2 variants of Bangladesh. We have also found four unique mutations from two of SARS-CoV-2 B.1.525 variant of Bangladesh. Among the four unique mutations two mutations (NS7a_L96H, NS7a_Y97D) obtained from strain BCSIR-NILMRC-718, one (NSP3_A1430V) from BCSIR-NILMRC-738 and two mutation including one spike protein mutation (NSP2_L444I, Spike_I68 M) present in BCSIR-AFIP-10 strain. The identification of new mutations will contribute to characterizing SARS-CoV-2, to continue tracking its spread and better understanding its biological and clinical features to take medical countermeasures and vaccines.

摘要

在本研究中，我们报告了来自孟加拉国的五株严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的完整基因组，这些病毒在刺突蛋白（E484K、Q677H、D614G、A67V、Q52R、Y144del、H69del、V70del、F888L）上发生了突变，被归为 B.1.525 谱系（关注变体）。除了刺突区域（E_L21F、M_I82F、N_A12G 和 N_T208I）之外，病毒结构蛋白中也发现了突变，以及来自所有五个孟加拉国 B.1.525 SARS-CoV-2 变体的其他突变（NSP3_T1189I、NSP6_S106del、NSP6_F108del、NSP6_G107del、NSP12_P323F）。我们还从孟加拉国的两个 SARS-CoV-2 B.1.525 变体中发现了四个独特的突变。在这四个独特的突变中，两个突变（NS7a_L96H、NS7a_Y97D）来自 BCSIR-NILMRC-718 株，一个（NSP3_A1430V）来自 BCSIR-NILMRC-738 株，而两个突变包括一个刺突蛋白突变（NSP2_L444I、Spike_I68M）存在于 BCSIR-AFIP-10 株中。新突变的鉴定将有助于表征 SARS-CoV-2，继续跟踪其传播，并更好地了解其生物学和临床特征，以采取医疗对策和疫苗。