College of General Education, Kookmin University, Seoul, South Korea.
Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong,18323, South Korea.
Food Chem. 2024 Mar 1;435:137548. doi: 10.1016/j.foodchem.2023.137548. Epub 2023 Sep 22.
This study investigated the impact of plasma-activated water (PAW) on Raphanus sativus (radish) roots at the level of proteins and metabolites. PAW treatment induced the accumulation of reactive oxygen species (ROS) and nitrogen oxide species (NO) in radish and enhanced the activities of antioxidant enzymes. Proteomic analysis resulted in the identification of 6054 proteins, including 1845 PAW-modulated proteins that were majorly associated with energy metabolism, ROS-detoxification, phytohormones signaling, and biosynthesis of glucosinolates. Subsequent metabolomics analysis identified 314 metabolites, of which 194 showed significant differences in response to PAW treatment. In particular, PAW treatment triggered the accumulation of functional compounds such as vitamin C, vitamin B5, glutathione, and glucosinolates, the well-known characteristic compounds of the Brassicaceae family. Further, integrating proteomics and metabolomics data provided novel insights into the molecular mechanism governing plasma-induced growth and the accumulation of these functional compounds in radish plants.
本研究调查了等离子体激活水（PAW）对萝卜根水平的蛋白质和代谢物的影响。PAW 处理诱导萝卜中活性氧（ROS）和氮氧化物（NO）的积累，并增强抗氧化酶的活性。蛋白质组学分析鉴定出 6054 种蛋白质，其中包括 1845 种与能量代谢、ROS 解毒、植物激素信号转导和硫代葡萄糖苷生物合成密切相关的 PAW 调节蛋白。随后的代谢组学分析鉴定出 314 种代谢物，其中 194 种对 PAW 处理表现出显著差异。特别是，PAW 处理触发了功能化合物的积累，如维生素 C、维生素 B5、谷胱甘肽和硫代葡萄糖苷，这是芸薹科植物的典型特征化合物。此外，整合蛋白质组学和代谢组学数据为等离子体诱导生长和萝卜植物中这些功能化合物积累的分子机制提供了新的见解。
