National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Jinan, 250100, People's Republic of China.
Planta. 2018 May;247(5):1109-1122. doi: 10.1007/s00425-018-2846-5. Epub 2018 Jan 24.
Differential abundance protein species (DAPS) involved in reducing damage and enhancing thermotolerance in radish were firstly identified. Proteomic analysis and omics association analysis revealed a HS-responsive regulatory network in radish. Heat stress (HS) is a major destructive factor influencing radish production and supply in summer, for radish is a cool season vegetable crop being susceptible to high temperature. In this study, the proteome changes of radish taproots under 40 °C treatment at 0 h (Control), 12 h (Heat12) and 24 h (Heat24) were analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) approach. In total, 2258 DAPS representing 1542 differentially accumulated uniprotein species which respond to HS were identified. A total of 604, 910 and 744 DAPS was detected in comparison of Control vs. Heat12, Control vs. Heat24, and Heat12 vs. Heat24, respectively. Gene ontology and pathway analysis showed that annexin, ubiquitin-conjugating enzyme, ATP synthase, heat shock protein (HSP) and other stress-related proteins were predominately enriched in signal transduction, stress and defense pathways, photosynthesis and energy metabolic pathways, working cooperatively to reduce stress-induced damage in radish. Based on iTRAQ combined with the transcriptomics analysis, a schematic model of a sequential HS-responsive regulatory network was proposed. The initial sensing of HS occurred at the plasma membrane, and then key components of stress signal transduction triggered heat-responsive genes in the plant protective metabolism to re-establish homeostasis and enhance thermotolerance. These results provide new insights into characteristics of HS-responsive DAPS and facilitate dissecting the molecular mechanisms underlying heat tolerance in radish and other root crops.
首次鉴定了参与萝卜减少损伤和增强耐热性的差异丰度蛋白种（DAPS）。蛋白质组学分析和组学关联分析揭示了萝卜中 HS 响应的调控网络。热应激（HS）是影响萝卜夏季生产和供应的主要破坏性因素，因为萝卜是一种喜凉的蔬菜作物，易受高温影响。在这项研究中，采用 iTRAQ（相对和绝对定量的同重同位素标记）方法分析了 40°C 处理 0 h（对照）、12 h（Heat12）和 24 h（Heat24）下萝卜肉质根的蛋白质组变化。总共鉴定出 2258 个 DAPS，代表了 1542 种对 HS 有反应的差异积累的单蛋白种。在对照与 Heat12、对照与 Heat24、Heat12 与 Heat24 的比较中，分别检测到 604、910 和 744 个 DAPS。GO 和途径分析表明， annexin、泛素连接酶、ATP 合酶、热休克蛋白（HSP）和其他应激相关蛋白主要富集在信号转导、应激和防御途径、光合作用和能量代谢途径中，共同作用于降低萝卜中的应激诱导损伤。基于 iTRAQ 结合转录组学分析，提出了一个顺序 HS 响应调控网络的示意图模型。HS 的初始感应发生在质膜上，然后应激信号转导的关键成分触发植物保护代谢中的热响应基因，以重新建立内稳态并增强耐热性。这些结果为 HS 响应 DAPS 的特征提供了新的见解，并有助于剖析萝卜和其他根作物耐热性的分子机制。
