Thet L A, Parra S C, Shelburne J D
Exp Lung Res. 1986;11(3):209-28. doi: 10.3109/01902148609064297.
We studied changes in lung ultrastructure and collagen content during the repair of acute lung injury in adult rats exposed to 100% O2 for 60 h and recovering in ambient air. In the interstitium, during the first 3 days of repair, the number of neutrophils decreased 16-fold, and monocytes and lymphocytes increased to 7-fold and 4-fold the respective control values. Myofibroblasts increased about 5-fold and the volume of the interstitial matrix remained high. By 7 days, the differential count of inflammatory cells was normal although the number of total interstitial cells and myofibroblasts decreased more slowly. In the capillary endothelium, after 3 days of repair, the cells were hypertrophied and had organelle-rich cytoplasm, and total cell number had increased back to control values; endothelial cell number increased an additional 63% between 3 and 7 days of repair. In the epithelium, type 2 cells increased 150% during the first 3 days of repair before decreasing; type 1 cell number did not change. After 28 days of repair, the lungs appeared qualitatively almost normal; however, interstitial cell number and collagen content were still increased. We conclude that the repair of oxygen-induced lung injury involves a complex pattern of morphologic changes that has important similarities to those occurring during repair on other tissues such as the skin.
我们研究了成年大鼠在暴露于100%氧气60小时并在环境空气中恢复过程中急性肺损伤修复期间肺超微结构和胶原蛋白含量的变化。在间质中，修复的前3天，中性粒细胞数量减少了16倍，单核细胞和淋巴细胞分别增加到各自对照值的7倍和4倍。肌成纤维细胞增加了约5倍，间质基质体积仍然很高。到第7天，炎症细胞的分类计数正常，尽管间质细胞总数和肌成纤维细胞数量下降得更慢。在毛细血管内皮中，修复3天后，细胞肥大，细胞质富含细胞器，细胞总数已恢复到对照值；内皮细胞数量在修复的第3天到第7天之间又增加了63%。在上皮中，2型细胞在修复的前3天增加了150%，然后减少；1型细胞数量没有变化。修复28天后，肺在质量上几乎正常；然而，间质细胞数量和胶原蛋白含量仍然增加。我们得出结论，氧诱导的肺损伤修复涉及复杂的形态学变化模式，这与皮肤等其他组织修复过程中发生的变化有重要的相似之处。
