OMIP-098：用于人类记忆 NK 细胞表型分析的 26 参数、24 色流式细胞术面板。
OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping.
机构信息
The US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA.
出版信息
Cytometry A. 2023 Dec;103(12):941-946. doi: 10.1002/cyto.a.24802. Epub 2023 Oct 8.
Abstract
This 26-parameter flow cytometry panel has been developed and optimized to analyze NK cell phenotype, using cryopreserved peripheral blood mononuclear cells (PBMCs) from people living with and without human immunodeficiency virus (PLWH, PWOH). Our panel is designed for the analysis of several parameters of total NK cells and memory NK cell subsets including markers of maturation, activation, and proliferation, as well as activating and inhibitory receptors. Other tissues have not been tested (Table 1).
摘要
本 26 参数量化流式细胞术面板是为分析人类免疫缺陷病毒（HIV）感染者和未感染者（PLWH 和 PWOH）的外周血单个核细胞（PBMC）中的 NK 细胞表型而开发和优化的。我们的面板设计用于分析总 NK 细胞和记忆 NK 细胞亚群的几个参数，包括成熟、激活和增殖标志物，以及激活和抑制受体。其他组织尚未进行测试（表 1）。
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