Li Meng, Chen Chuanjie, Zhang Haiyang, Wang Zongshuai, Song Ningning, Li Junlin, Liang Xiaoyan, Yi Kuihua, Gu Yinyu, Guo Xiaohong
Shandong Institute of Sericulture, Shandong Academy of Agricultural Sciences, Yantai, China.
Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
Front Microbiol. 2023 Sep 22;14:1250453. doi: 10.3389/fmicb.2023.1250453. eCollection 2023.
The biochar and organic fertilizer amendment have been used as an effective practice to increase soil fertility. Nevertheless, the mechanisms of microbial community response to organic fertilizer and biochar application on saline-alkali soil have not been clarified. This study investigated the effects at different concentrations of organic fertilizer and biochar on the microbial community of wheat rhizosphere soil under field experiment in the Yellow River Delta (China, YRD), using high-throughput sequencing technology. Biochar and organic fertilizer significantly influenced in most soil parameters ( < 0.05), apart from soil moisture content (M), pH, total nitrogen (TN) and soil total phosphorus (TP). Proteobacteria and Actinobacteriota were found in the rhizosphere soil as the main bacterial phyla, and the main fungal phyla were Ascomycota and Mortierellomycota. The soil bacterial and fungal communities under organic fertilizer were distinct from CK. Furthermore, redundancy analysis (RDA) directed that changes in bacterial communities were related to soil properties like pH, available phosphorus (AP), and total organic carbon (TOC), while pH, AP and TP, were crucial contributors in regulating fungal distribution. The correlation between soil parameters and bacteria or fungi varied with the application of biochar and organic fertilizers, and the interaction between the bacteria and fungi in organic fertilizer treatments formed more connections compared with biochar treatments. Our results indicated that biochar was superior to organic fertilizer under the contents set up in this study, and soil parameters increased with biochar and organic fertilizer application rate. The diversity and structure of soil bacteria and fungi differed with the application of biochar and organic fertilizer. The research provides a reference to rational application of organic fertilizer and biochar improvement in saline-alkali soil.
生物炭和有机肥改良已被用作提高土壤肥力的有效措施。然而，盐碱土上微生物群落对有机肥和生物炭施用的响应机制尚未阐明。本研究采用高通量测序技术，在黄河三角洲（中国，YRD）的田间试验中，研究了不同浓度的有机肥和生物炭对小麦根际土壤微生物群落的影响。除土壤含水量（M）、pH值、全氮（TN）和土壤总磷（TP）外，生物炭和有机肥对大多数土壤参数均有显著影响（<0.05）。在根际土壤中发现变形菌门和放线菌门是主要细菌门类，主要真菌门类是子囊菌门和被孢霉门。有机肥处理下的土壤细菌和真菌群落与对照不同。此外，冗余分析（RDA）表明，细菌群落的变化与土壤性质如pH值、有效磷（AP）和总有机碳（TOC）有关，而pH值、AP和TP是调节真菌分布的关键因素。土壤参数与细菌或真菌之间的相关性随生物炭和有机肥的施用而变化，与生物炭处理相比，有机肥处理中细菌和真菌之间的相互作用形成了更多的联系。我们的结果表明，在本研究设定的含量下，生物炭优于有机肥，土壤参数随生物炭和有机肥施用量的增加而增加。土壤细菌和真菌的多样性和结构因生物炭和有机肥的施用而不同。该研究为盐碱土上有机肥和生物炭改良的合理施用提供了参考。
