Crean E V, Tyson S L, Richardson T M
Exp Eye Res. 1986 Sep;43(3):365-74. doi: 10.1016/s0014-4835(86)80073-2.
When cell or explant cultures were established from excised calf aqueous outflow pathway tissue and subsequently labeled with radioactive precursors of glycosaminoglycans [( 3H]glucosamine and [35S]sulfate), hyaluronic acid was the predominant glycosaminoglycan produced initially. As the cultures reached confluence, sulfated glycosaminoglycans became more prominent products. Further investigation revealed that the cultured trabecular cells could synthesize all of the glycosaminoglycans found in isolated calf trabecular tissue once a critical cell density had been attained. Increasing concentrations of fetal bovine serum in the culture medium stimulated glycosaminoglycan accumulation without altering the quantitative distribution of the various glycosaminoglycan products. Treatment of confluent monolayers with enzymes capable of degrading extracellular matrix molecules caused marked changes in the distribution of glycosaminoglycans produced by calf trabecular-cell cultures. The results suggest that calf trabecular cell cultures can be used to study the dynamics of extracellular matrix production and turnover under controlled experimental conditions.
当从小牛房水流出途径组织中切除的组织建立细胞或外植体培养物，并随后用糖胺聚糖的放射性前体（[3H]葡糖胺和[35S]硫酸盐）进行标记时，透明质酸是最初产生的主要糖胺聚糖。随着培养物达到汇合状态，硫酸化糖胺聚糖成为更突出的产物。进一步的研究表明，一旦达到临界细胞密度，培养的小梁细胞就能合成在分离的小牛小梁组织中发现的所有糖胺聚糖。培养基中胎牛血清浓度的增加刺激了糖胺聚糖的积累，而不改变各种糖胺聚糖产物的定量分布。用能够降解细胞外基质分子的酶处理汇合的单层细胞，导致小牛小梁细胞培养物产生的糖胺聚糖分布发生显著变化。结果表明，小牛小梁细胞培养物可用于在可控实验条件下研究细胞外基质产生和周转的动力学。
