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鱼类 STAT 基因的进化、比较和功能分析及其在细菌和 Poly(I:C)刺激下 JAK-STAT 通路的调控

Evolutionary, comparative, and functional analyses of STATs and regulation of the JAK-STAT pathway in lumpfish upon bacterial and poly(I:C) exposure.

机构信息

Department of Biological Sciences, Bergen High-Technology Centre, University of Bergen, Bergen, Norway.

出版信息

Front Cell Infect Microbiol. 2023 Sep 22;13:1252744. doi: 10.3389/fcimb.2023.1252744. eCollection 2023.

DOI:10.3389/fcimb.2023.1252744
PMID:37808912
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10556531/
Abstract

BACKGROUND

The Janus kinase/signal transducers and activators of transcription (JAK-STAT) system regulates several biological processes by affecting transcription of genes as a response to cytokines and growth factors. In the present study, we have characterized the STAT genes in lumpfish ( L.), belonging to the order Perciformes, and investigated regulation of the JAK-STAT signaling pathway upon exposure to bacteria () and poly(I:C), the latter mimicking antiviral responses.

METHODS

Characterization and evolutionary analyses of the STATs were performed by phylogeny, protein domain, homology similarity and synteny analyses. Antibacterial and antiviral responses were investigated by performing KEGG pathway analysis.

RESULTS

We observed that lumpfish have , , , , , , and . Transcriptome-wide analyses showed that most components of the JAK-STAT pathway were present in lumpfish. -6, -10, -21, and were upregulated 6 hours post exposure (hpe) against bacteria while type I interferons (IFNs), , , , and were upregulated 24 hpe against poly(I:C).

CONCLUSIONS

Our findings shed light on the diversity and evolution of the STATs and the data show that the STAT genes are highly conserved among fish, including lumpfish. The transcriptome-wide analyses lay the groundwork for future research into the functional significance of these genes in regulating critical biological processes and make an important basis for development of prophylactic measure such as vaccination, which is highly needed for lumpfish since it is vulnerable for both bacterial and viral diseases.

摘要

背景

Janus 激酶/信号转导与转录激活因子（JAK-STAT）系统通过影响细胞因子和生长因子反应中的基因转录来调节多种生物过程。在本研究中，我们对鲈形目鱼类的 STAT 基因进行了特征描述，并研究了 JAK-STAT 信号通路在细菌（）和 poly（I:C）暴露下的调控，后者模拟抗病毒反应。

方法

通过系统发育、蛋白质结构域、同源性相似性和基因同线性分析对 STATs 进行了特征描述和进化分析。通过进行 KEGG 通路分析，研究了抗菌和抗病毒反应。

结果

我们观察到，褐菖鲉具有、、、、、和。转录组分析表明，褐菖鲉 JAK-STAT 通路的大多数组成部分都存在。在细菌暴露 6 小时后，-6、-10、-21、和上调，而 I 型干扰素（IFNs）、、、、和在 poly（I:C）暴露 24 小时后上调。

结论

我们的研究结果揭示了 STATs 的多样性和进化，并且数据表明 STAT 基因在鱼类中高度保守，包括褐菖鲉。转录组分析为进一步研究这些基因在调节关键生物学过程中的功能意义奠定了基础，并为预防措施的开发提供了重要依据，由于褐菖鲉易患细菌和病毒疾病，因此非常需要这种预防措施，例如疫苗接种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/f03afff222f9/fcimb-13-1252744-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/cc86d914e2a2/fcimb-13-1252744-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/eb2362541dbb/fcimb-13-1252744-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/c310408dcf35/fcimb-13-1252744-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/82bf816539ed/fcimb-13-1252744-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/f03afff222f9/fcimb-13-1252744-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/cc86d914e2a2/fcimb-13-1252744-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/eb2362541dbb/fcimb-13-1252744-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/c310408dcf35/fcimb-13-1252744-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/82bf816539ed/fcimb-13-1252744-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc2f/10556531/f03afff222f9/fcimb-13-1252744-g005.jpg

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5
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6
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